Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons
المؤلفون المشاركون
Duong, Thu T.
Lim, James
Vasireddy, Vidyullatha
Papp, Tyler
Nguyen, Hung
Leo, Lanfranco
Pan, Jieyan
Zhou, Shangzhen
Chen, H. Isaac
Bennett, Jean
Mills, Jason A.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-17
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Recombinant adeno-associated virus (rAAV), produced from a nonpathogenic parvovirus, has become an increasing popular vector for gene therapy applications in human clinical trials.
However, transduction and transgene expression of rAAVs can differ across in vitro and ex vivo cellular transduction strategies.
This study compared 11 rAAV serotypes, carrying one reporter transgene cassette containing a cytomegalovirus immediate-early enhancer (eCMV) and chicken beta actin (CBA) promoter driving the expression of an enhanced green-fluorescent protein (eGFP) gene, which was transduced into four different cell types: human iPSC, iPSC-derived RPE, iPSC-derived cortical, and dissociated embryonic day 18 rat cortical neurons.
Each cell type was exposed to three multiplicity of infections (MOI: 1E4, 1E5, and 1E6 vg/cell).
After 24, 48, 72, and 96 h posttransduction, GFP-expressing cells were examined and compared across dosage, time, and cell type.
Retinal pigmented epithelium showed highest AAV-eGFP expression and iPSC cortical the lowest.
At an MOI of 1E6 vg/cell, all serotypes show measurable levels of AAV-eGFP expression; moreover, AAV7m8 and AAV6 perform best across MOI and cell type.
We conclude that serotype tropism is not only capsid dependent but also cell type plays a significant role in transgene expression dynamics.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duong, Thu T.& Lim, James& Vasireddy, Vidyullatha& Papp, Tyler& Nguyen, Hung& Leo, Lanfranco…[et al.]. 2019. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209529
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duong, Thu T.…[et al.]. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1209529
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duong, Thu T.& Lim, James& Vasireddy, Vidyullatha& Papp, Tyler& Nguyen, Hung& Leo, Lanfranco…[et al.]. Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209529
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209529
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر