![](/images/graphics-bg.png)
Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors
المؤلفون المشاركون
Schmelzer, Eva
Gerlach, Joerg C.
Thompson, Robert L.
Gridelli, Bruno
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-18
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
Although the hepatic and hematopoietic progenitors of the liver are well characterized, the interactions between these two lineages remain mostly elusive.
Hepatoblasts express delta-like noncanonical Notch ligand 1 (Dlk1), whose cleaved extracellular domain can become a soluble protein.
We assessed the effects of DLK1 gene expression knockdown in cultures of total fetal liver cells.
Furthermore, we separated Dlk1+ hepatoblasts from the total liver cell fraction and investigated effects of direct cell contact.
Dlk1- cells were cultured either without Dlk1+ hepatoblasts, in direct contact with hepatoblasts, or separated from hepatoblasts by a porous membrane in inserts to inhibit cell contact but allow free exchange of molecules.
Expression of the hepatic and hematopoietic genes, colony forming unit potential of various hematopoietic progenitors, and cell numbers and types were investigated.
We found that DLK1 knockdown in total fetal liver cell cultures decreased total cell numbers.
The expression of hepatic progenitor genes and mature hematopoietic genes was affected.
Hematopoietic BFU-E and CFU-GM colony numbers were reduced significantly.
The depletion of Dlk1+ hepatoblasts in culture decreased the potential of all hematopoietic progenitors to form colonies of all types and reduced the percentage of mature hematopoietic cells.
The addition of hepatoblasts in inserts to Dlk1- cells further decreased the potential to form the CFU-GM and CFU-GEMM colonies and the percentage of mature hematopoietic cells but increased total cell numbers.
Conclusively, direct contact of Dlk1 supports hematopoietic progenitor expansion and functionality that cannot be reconstituted in coculture without direct cell contact.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gerlach, Joerg C.& Thompson, Robert L.& Gridelli, Bruno& Schmelzer, Eva. 2019. Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors. Stem Cells International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1209613
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gerlach, Joerg C.…[et al.]. Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors. Stem Cells International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1209613
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gerlach, Joerg C.& Thompson, Robert L.& Gridelli, Bruno& Schmelzer, Eva. Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1209613
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209613
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)