Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia
المؤلفون المشاركون
Pacienza, Natalia
Santa-Cruz, Diego
Malvicini, Ricardo
Robledo, Oscar
Lemus-Larralde, Gastón
Bertolotti, Alejandro
Marcos, Martín
Yannarelli, Gustavo
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-05
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Lung transplantation is a lifesaving therapy for people living with severe, life-threatening lung disease.
The high mortality rate among patients awaiting transplantation is mainly due to the low percentage of lungs that are deemed acceptable for implantation.
Thus, the current shortage of lung donors may be significantly reduced by implementing different therapeutic strategies which facilitate both organ preservation and recovery.
Here, we studied whether the anti-inflammatory effect of human umbilical cord-derived mesenchymal stem cells (HUCPVCs) increases lung availability by improving organ preservation.
We developed a lung preservation rat model that mimics the different stages by which donor organs must undergo before implantation.
The therapeutic schema was as follows: cardiac arrest, warm ischemia (2 h at room temperature), cold ischemia (1.5 h at 4°C, with Perfadex), and normothermic lung perfusion with ventilation (Steen solution, 1 h).
After 1 h of warm ischemia, HUCPVCs (1×106 cells) or vehicle was infused via the pulmonary artery.
Physiologic data (pressure-volume curves) were acquired right after the cardiac arrest and at the end of the perfusion.
Interestingly, although lung edema did not change among groups, lung compliance dropped to 34% in the HUCPVC-treated group, while the vehicle group showed a stronger reduction (69%, p<0.0001).
Histologic assessment demonstrated less overall inflammation in the HUCPVC-treated lungs.
In addition, MPO activity, a neutrophil marker, was reduced by 41% compared with vehicle (p<0.01).
MSC therapy significantly decreased tissue oxidative damage by controlling reactive oxygen species production.
Accordingly, catalase and superoxide dismutase enzyme activities remained at baseline levels.
In conclusion, these results demonstrate that the anti-inflammatory effect of MSCs protects donor lungs against ischemic injury and postulates MSC therapy as a novel tool for organ preservation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pacienza, Natalia& Santa-Cruz, Diego& Malvicini, Ricardo& Robledo, Oscar& Lemus-Larralde, Gastón& Bertolotti, Alejandro…[et al.]. 2019. Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209633
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pacienza, Natalia…[et al.]. Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209633
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pacienza, Natalia& Santa-Cruz, Diego& Malvicini, Ricardo& Robledo, Oscar& Lemus-Larralde, Gastón& Bertolotti, Alejandro…[et al.]. Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209633
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209633
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر