Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells
المؤلفون المشاركون
Nguyen, Thi Minh Xuan
Vegrichtova, Marketa
Tlapakova, Tereza
Krulova, Magdalena
Krylov, Vladimir
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-05
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development by which sessile epithelial cells are converted into migratory mesenchymal cells.
Our laboratory has been successful in the establishment of Xenopus tropicalis immature Sertoli cells (XtiSCs) with the restricted differentiation potential.
The aim of this study is the determination of factors responsible for EMT activation in XtiSCs and stemness window acquisition where cells possess the broadest differentiation potential.
For this purpose, we tested three potent EMT inducers—GSK-3 inhibitor (CHIR99021), FGF2, and/or TGF-β1 ligand.
XtiSCs underwent full EMT after 3-day treatment with CHIR99021 and partial EMT with FGF2 but not with TGF-β1.
The morphological change of CHIR-treated XtiSCs to the typical spindle-like cell shape was associated with the upregulation of mesenchymal markers and the downregulation of epithelial markers.
Moreover, only CHIR-treated XtiSCs were able to differentiate into chondrocytes in vitro and cardiomyocytes in vivo.
Interestingly, EMT-shifted cells could migrate towards cancer cells (HeLa) in vitro and to the injury site in vivo.
The results provide a better understanding of signaling pathways underlying the generation of testis-derived stem cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nguyen, Thi Minh Xuan& Vegrichtova, Marketa& Tlapakova, Tereza& Krulova, Magdalena& Krylov, Vladimir. 2019. Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209699
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nguyen, Thi Minh Xuan…[et al.]. Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells. Stem Cells International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1209699
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nguyen, Thi Minh Xuan& Vegrichtova, Marketa& Tlapakova, Tereza& Krulova, Magdalena& Krylov, Vladimir. Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209699
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209699
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر