Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats
المؤلفون المشاركون
Wang, Feng
Nan, Li-ping
Zhou, Shi-feng
Liu, Yang
Wang, Ze-yu
Wang, Jing-cheng
Feng, Xin-min
Zhang, Liang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-15
دولة النشر
مصر
عدد الصفحات
17
الملخص EN
Stem cell-based tissue engineering in treating intervertebral disc (IVD) degeneration is promising.
An appropriate cell scaffold can maintain the viability and function of transplanted cells.
Injectable hydrogel has the potential to be an appropriate cell scaffold as it can mimic the condition of the natural extracellular matrix (ECM) of nucleus pulposus (NP) and provide binding sites for cells.
This study was aimed at investigating the effect of injectable hydrogel-loaded NP-derived mesenchymal stem cells (NPMSC) for the treatment of IVD degeneration (IDD) in rats.
In this study, we selected injectable 3D-RGD peptide-modified polysaccharide hydrogel as a cell transplantation scaffold.
In vitro, the biocompatibility, microstructure, and induced differentiation effect on NPMSC of the hydrogel were studied.
In vivo, the regenerative effect of hydrogel-loaded NPMSC on degenerated NP in a rat model was evaluated.
The results showed that NPMSC was biocompatible and able to induce differentiation in hydrogel in vivo.
The disc height index (almost 87%) and MRI index (3313.83±227.79) of the hydrogel-loaded NPMSC group were significantly higher than those of other groups at 8 weeks after injection.
Histological staining and immunofluorescence showed that the hydrogel-loaded NPMSC also partly restored the structure and ECM content of degenerated NP after 8 weeks.
Moreover, the hydrogel could support long-term NPMSC survival and decrease cell apoptosis rate of the rat IVD.
In conclusion, injectable hydrogel-loaded NPMSC transplantation can delay the level of IDD and promote the regeneration of the degenerative IVD in the rat model.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Feng& Nan, Li-ping& Zhou, Shi-feng& Liu, Yang& Wang, Ze-yu& Wang, Jing-cheng…[et al.]. 2019. Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats. Stem Cells International،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209765
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Feng…[et al.]. Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats. Stem Cells International No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1209765
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Feng& Nan, Li-ping& Zhou, Shi-feng& Liu, Yang& Wang, Ze-yu& Wang, Jing-cheng…[et al.]. Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209765
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209765
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر