Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro
المؤلفون المشاركون
Woo, So-Yeon
Park, Minhwa
Kim, Yu-Hee
Ryu, Kyung-Ha
Cho, Kyung-Ah
Lee, Hyun-Ji
Kim, Han Su
Park, Joo-Won
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-10
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
The application of mesenchymal stem cells (MSCs) for treating bone-related diseases shows promising outcomes in preclinical studies.
However, cells that are isolated and defined as MSCs comprise a heterogeneous population of progenitors.
This heterogeneity can produce variations in the performance of MSCs, especially in applications that require differentiation potential in vivo, such as the treatment of osteoporosis.
Here, we aimed to identify genetic markers in tonsil-derived MSCs (T-MSCs) that can predict osteogenic potential.
Using a single-cell cloning method, we isolated and established several lines of nondifferentiating (ND) or osteoblast-prone (OP) clones.
Next, we performed transcriptome sequencing of three ND and three OP clones that maintained the characteristics of MSCs and determined the top six genes that were upregulated in OP clones.
Upregulation of WNT16 and DCLK1 expression was confirmed by real-time quantitative PCR, but only WNT16 expression was correlated with the osteogenic differentiation of T-MSCs from 10 different donors.
Collectively, our findings suggest that WNT16 is a putative genetic marker that predicts the osteogenic potential of T-MSCs.
Thus, examination of WNT16 expression as a selection criterion prior to the clinical application of MSCs may enhance the therapeutic efficacy of stem cell therapy for bone-related complications, including osteoporosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Yu-Hee& Cho, Kyung-Ah& Lee, Hyun-Ji& Park, Minhwa& Kim, Han Su& Park, Joo-Won…[et al.]. 2019. Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro. Stem Cells International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1209770
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Yu-Hee…[et al.]. Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro. Stem Cells International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1209770
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Yu-Hee& Cho, Kyung-Ah& Lee, Hyun-Ji& Park, Minhwa& Kim, Han Su& Park, Joo-Won…[et al.]. Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1209770
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209770
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر