Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway
المؤلفون المشاركون
Ji, Shangli
Xiao, Jie
Liu, Jian
Tang, Shibo
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-15
دولة النشر
مصر
عدد الصفحات
17
الملخص EN
Glaucoma is characterized by progressive, irreversible damage to the retinal ganglion cells (RGCs) and their axons.
Our previous study has shown that the intravitreal transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) reveals a neuroprotective role in microsphere injection-induced ocular hypertension (OHT) rat models.
The protection is related to the modulation of glial cells, but the mechanisms are still unknown.
The purpose of the present study is to clarify the potential neuroinflammatory mechanisms involved in the neuroprotective role of hUC-MSCs.
OHT models were established with SD rats through intracameral injection of polystyrene microbeads.
The animals were randomly divided into three groups: the normal group, the OHT+phosphate-buffered saline (PBS) group, and the OHT+hUC-MSC group.
Retinal morphology was evaluated by measuring the inner retinal thickness via optical coherence tomography (OCT).
Retinal cell apoptosis was examined by TUNEL staining and Bax expression 14 days following hUC-MSC transplantation.
The expression levels of glial fibrillary acidic protein (GFAP), ionized calcium binding adapter molecule 1 (iba-1), and toll-like receptor 4 (TLR4) were assessed via immunohistochemistry, real-time quantitative PCR, and Western blot.
RNA and proteins were extracted 14 days following transplantation, and the expression levels of the TLR4 signaling pathways and proinflammatory cytokines—myeloid differentiation factor 88 (MyD88), IL-1β, IL-6, and TNF-α—were determined.
OCT showed that the intravitreal transplantation of hUC-MSCs significantly increased the inner thickness of the retina.
A TUNEL assay and the expression of Bax suggested that the apoptosis of retinal cells was decreased by hUC-MSCs 14 days following transplantation.
Intravitreal hUC-MSC transplantation resulted in a decreased expression of GFAP, iba-1, TLR4, MyD88, IL-1β, IL-6, and TNF-α 14 days following transplantation.
In addition, via in vitro experiments, we found that the increased expression of the TLR4 signaling pathway induced by lipopolysaccharide (LPS) was markedly decreased after hUC-MSCs were cocultured with rMC-1 and BV2 cells.
These findings indicate that hUC-MSC transplantation attenuates OHT-induced retinal neuroinflammation via the TLR4 pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ji, Shangli& Xiao, Jie& Liu, Jian& Tang, Shibo. 2019. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway. Stem Cells International،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209948
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ji, Shangli…[et al.]. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway. Stem Cells International No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1209948
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ji, Shangli& Xiao, Jie& Liu, Jian& Tang, Shibo. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209948
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209948
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر