Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment
المؤلفون المشاركون
Chapman, Joab
Maggio, Nicola
Ben Shimon, Marina
Shavit Stein, Efrat
Artan Furman, Avital
Golderman, Valery
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Systemic inflammation and brain pathologies are known to be linked.
In the periphery, the inflammation and coagulation systems are simultaneously activated upon diseases and infections.
Whether this well-established interrelation also counts for neuroinflammation and coagulation factor expression in the brain is still an open question.
Our aim was to study whether the interrelationship between coagulation and inflammation factors may occur in the brain in the setting of systemic inflammation.
The results indicate that systemic injections of lipopolysaccharide (LPS) upregulate the expression of both inflammatory and coagulation factors in the brain.
The activity of the central coagulation factor thrombin was tested by a fluorescent method and found to be significantly elevated in the hippocampus following systemic LPS injection (0.5 ± 0.15 mU/mg versus 0.2 ± 0.03 mU/mg in the control).
A panel of coagulation factors and effectors (such as thrombin, FX, PAR1, EPCR, and PC) was tested in the hippocampus, isolated microglia, and N9 microglia cell by Western blot and real-time PCR and found to be modulated by LPS.
One central finding is a significant increase in FX expression level following LPS induction both in vivo in the hippocampus and in vitro in N9 microglia cell line (5.5 ± 0.6- and 2.3 ± 0.1-fold of increase, resp.).
Surprisingly, inhibition of thrombin activity (by a specific inhibitor NAPAP) immediately after LPS injection results in a reduction of both the inflammatory (TNFα, CXL9, and CCL1; p<0.006) and coagulation responses (FX and PAR1; p<0.004) in the brain.
We believe that these results may have a profound clinical impact as they might indicate that reducing coagulation activity in the setting of neurological diseases involving neuroinflammation may improve disease outcome and survival.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shavit Stein, Efrat& Ben Shimon, Marina& Artan Furman, Avital& Golderman, Valery& Chapman, Joab& Maggio, Nicola. 2018. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment. Neural Plasticity،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1210372
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shavit Stein, Efrat…[et al.]. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment. Neural Plasticity No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1210372
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shavit Stein, Efrat& Ben Shimon, Marina& Artan Furman, Avital& Golderman, Valery& Chapman, Joab& Maggio, Nicola. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment. Neural Plasticity. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1210372
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1210372
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر