Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells
المؤلفون المشاركون
Yang, Nae-Cherng
Huang-Liu, Rosa
Chang, Yuan-Jhe
Chang, Ya-Chun
Chen, Chia-Wen
Lee, Inn
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-10
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
This study is aimed at developing a method that can inhibit resveratrol (Res) degradation in Dulbecco’s modified Eagle medium (DMEM) and at evaluating the effects of Res on the replicative lifespan of Hs68 cells.
We hypothesized that Res can extend the lifespan of Hs68 cells if we can inhibit the oxidative degradation of Res in the medium.
We found that the addition of ≥5 U/mL SOD to the medium could completely inhibit Res degradation in DMEM.
Fetal bovine serum (FBS) contained 29.3 ± 1.1 U/mL of SOD activity.
FBS could prevent Res degradation in the medium through SOD activity and Res–FBS interaction, but the regular FBS concentration (i.e., 10% FBS) exhibited an insufficient ability to completely inhibit Res degradation.
We found that pyruvate (1 mM) could potentiate SOD to scavenge superoxide at approximately 2.2-fold.
Thus, 10% FBS combined with pyruvate (1 mM) could completely inhibit Res degradation.
When Res was not degraded, it still shortened the lifespan of Hs68 cells.
Overall, the proposed method involving 10% FBS combined with pyruvate (1 mM) could completely prevent Res degradation.
However, in contrast to our hypothesis, Res could induce the shortening of the lifespan of Hs68 cells.
The stability of Res analogs (i.e., oxy-Res and acetyl-Res) in the medium and their effects on the lifespan of Hs68 cells were also investigated.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chang, Yuan-Jhe& Chang, Ya-Chun& Huang-Liu, Rosa& Chen, Chia-Wen& Lee, Inn& Yang, Nae-Cherng. 2018. Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211011
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chang, Yuan-Jhe…[et al.]. Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1211011
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chang, Yuan-Jhe& Chang, Ya-Chun& Huang-Liu, Rosa& Chen, Chia-Wen& Lee, Inn& Yang, Nae-Cherng. Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211011
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211011
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر