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C60 Fullerene Prevents Restraint Stress-Induced Oxidative Disorders in Rat Tissues: Possible Involvement of the Nrf2ARE-Antioxidant Pathway
المؤلفون المشاركون
Prylutskyy, Yuriy
Gonchar, Olga O.
Maznychenko, Andriy V.
Bulgakova, Nataliya V.
Vereshchaka, Inna V.
Tomiak, Tomasz
Ritter, Uwe
Mankovska, Iryna M.
Kostyukov, Alexander I.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-13
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
The effects of C60FAS (50 and 500 μg/kg) supplementation, in a normal physiological state and after restraint stress exposure, on prooxidant/antioxidant balance in rat tissues were explored and compared with the effects of the known exogenous antioxidant N-acetylcysteine.
Oxidative stress biomarkers (ROS, O2⋅−, H2O2, and lipid peroxidation) and indices of antioxidant status (MnSOD, catalase, GPx, GST, γ-GCL, GR activities, and GSH level) were measured in the brain and the heart.
In addition, protein expression of Nrf2 in the nuclear and cytosol fractions as well as the protein level of antiradical enzyme MnSOD and GSH-related enzymes γ-GCLC, GPx, and GSTP as downstream targets of Nrf2 was evaluated by western blot analysis.
Under a stress condition, C60FAS attenuates ROS generation and O2⋅− and H2O2 releases and thus decreases lipid peroxidation as well as increases rat tissue antioxidant capacity.
We have shown that C60FAS supplementation has dose-dependent and tissue-specific effects.
C60FAS strengthened the antiradical defense through the upregulation of MnSOD in brain cells and maintained MnSOD protein content at the control level in the myocardium.
Moreover, C60FAS enhanced the GSH level and the activity/protein expression of GSH-related enzymes.
Correlation of these changes with Nrf2 protein content suggests that under stress exposure, along with other mechanisms, the Nrf2/ARE-antioxidant pathway may be involved in regulation of glutathione homeostasis.
In our study, in an in vivo model, when C60FAS (50 and 500 μg/kg) was applied alone, no significant changes in Nrf2 protein expression as well as in activity/protein levels of MnSOD and GSH-related enzymes in both tissues types were observed.
All these facts allow us to assume that in the in vivo model, C60FAS affects on the brain and heart endogenous antioxidative statuses only during the oxidative stress condition.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gonchar, Olga O.& Maznychenko, Andriy V.& Bulgakova, Nataliya V.& Vereshchaka, Inna V.& Tomiak, Tomasz& Ritter, Uwe…[et al.]. 2018. C60 Fullerene Prevents Restraint Stress-Induced Oxidative Disorders in Rat Tissues: Possible Involvement of the Nrf2ARE-Antioxidant Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1211060
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gonchar, Olga O.…[et al.]. C60 Fullerene Prevents Restraint Stress-Induced Oxidative Disorders in Rat Tissues: Possible Involvement of the Nrf2ARE-Antioxidant Pathway. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1211060
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gonchar, Olga O.& Maznychenko, Andriy V.& Bulgakova, Nataliya V.& Vereshchaka, Inna V.& Tomiak, Tomasz& Ritter, Uwe…[et al.]. C60 Fullerene Prevents Restraint Stress-Induced Oxidative Disorders in Rat Tissues: Possible Involvement of the Nrf2ARE-Antioxidant Pathway. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1211060
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211060
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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