Mitofusin2 Induces Cell Autophagy of Pancreatic Cancer through Inhibiting the PI3KAktmTOR Signaling Pathway
المؤلفون المشاركون
Xue, Ran
Hao, Jianyu
Liu, Xinjuan
Wang, Yanbin
Meng, Qinghua
Lu, Di
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-26
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Aim.
Pancreatic cancer is one of the most quickly fatal cancers around the world.
Burgeoning researches have begun to prove that mitochondria play a crucial role in cancer treatment.
Mitofusin2 (Mfn2) plays an indispensable role in mitochondrial fusion and adjusting function.
However, the role and underlying mechanisms of Mfn2 on cell autophagy of pancreatic cancer is still unclear.
Our aim was to explore the effect of Mfn2 on multiple biological functions involving cell autophagy in pancreatic cancer.
Methods.
Pancreatic cancer cell line, Aspc-1, was treated with Ad-Mfn2 overexpression.
Western blotting, caspase-3 activity measurement, and CCK-8 and reactive oxygen species (ROS) assay were used to examine the effects of Mfn2 on pancreatic cancer autophagy, apoptosis, cell proliferation, oxidative stress, and PI3K/Akt/mTOR signaling.
The expression of tissue Mfn2 was detected by immunohistochemical staining.
Survival analysis of Mfn2 was evaluated by OncoLnc.
Results.
Mfn2 improved the expression of LC3-II and Bax and downregulated the expression of P62 and Bcl-2 in pancreatic cancer cells.
Meanwhile, Mfn2 also significantly inhibited the expression of p-PI3K, p-Akt, and p-mTOR proteins in pancreatic cancer cells.
In addition, Mfn2 inhibited pancreatic cancer cell proliferation and ROS production.
Assessment of Kaplan-Meier curves showed that Mfn2− pancreatic cancer has a worse prognosis than Mfn2+ pancreatic cancer has.
Conclusions.
Our finding suggests that Mfn2 induces cell autophagy of pancreatic cancer through inhibiting the PI3K/Akt/mTOR signaling pathway.
Meanwhile, Mfn2 also influences multiple biological functions of pancreatic cancer cells.
Mfn2 may act as a therapeutic target in pancreatic cancer treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xue, Ran& Meng, Qinghua& Lu, Di& Liu, Xinjuan& Wang, Yanbin& Hao, Jianyu. 2018. Mitofusin2 Induces Cell Autophagy of Pancreatic Cancer through Inhibiting the PI3KAktmTOR Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1211095
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xue, Ran…[et al.]. Mitofusin2 Induces Cell Autophagy of Pancreatic Cancer through Inhibiting the PI3KAktmTOR Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1211095
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xue, Ran& Meng, Qinghua& Lu, Di& Liu, Xinjuan& Wang, Yanbin& Hao, Jianyu. Mitofusin2 Induces Cell Autophagy of Pancreatic Cancer through Inhibiting the PI3KAktmTOR Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1211095
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211095
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر