Increased Mitochondrial Protein Levels and Bioenergetics in the Musculus Rectus Femoris of Wfs1-Deficient Mice
المؤلفون المشاركون
Kõks, Sulev
Eimre, Margus
Peet, Nadežda
Kadaja, Lumme
Paju, Kalju
Tarrend, Marian
Kasvandik, Sergo
Seppet, Joosep
Ivask, Marilin
Orlova, Ehte
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-21
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Wfs1 deficiency leads to a progressive loss of plasma insulin concentration, which should reduce the consumption of glucose in insulin-dependent tissues, causing a variety of changes in intracellular energy metabolism.
Our objective here was to assess the changes in the amount and function of mitochondrial proteins in different muscles of Wfs1-deficient mice.
Mitochondrial functions were assayed by high-resolution oxygraphy of permeabilized muscle fibers; the protein amount was evaluated by liquid chromatography tandem mass spectrometry (LC/MS/MS) analysis and mRNA levels of the uncoupler proteins UCP2 and UCP3 by real-time PCR; and citrate synthase (CS) activity was determined spectrophotometrically in muscle homogenates.
Compared to controls, there were no changes in proton leak and citrate synthase activity in the heart and m.
soleus tissues of Wfs1-deficient mice, but significantly higher levels of both of these factors were observed in the m.
rectus femoris; mitochondrial proteins and mRNA of UCP2 were also higher in the m.
rectus femoris.
ADP-stimulated state 3 respiration was lower in the m.
soleus, remained unchanged in the heart, and was higher in the m.
rectus femoris.
The mitochondrial protein amount and activity are higher in Wfs1-deficient mice, as are mitochondrial proton leak and oxygen consumption in m.
rectus femoris.
These changes in muscle metabolism may be important for identifying the mechanisms responsible for Wolfram syndrome and diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Eimre, Margus& Paju, Kalju& Peet, Nadežda& Kadaja, Lumme& Tarrend, Marian& Kasvandik, Sergo…[et al.]. 2018. Increased Mitochondrial Protein Levels and Bioenergetics in the Musculus Rectus Femoris of Wfs1-Deficient Mice. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211181
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Eimre, Margus…[et al.]. Increased Mitochondrial Protein Levels and Bioenergetics in the Musculus Rectus Femoris of Wfs1-Deficient Mice. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211181
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Eimre, Margus& Paju, Kalju& Peet, Nadežda& Kadaja, Lumme& Tarrend, Marian& Kasvandik, Sergo…[et al.]. Increased Mitochondrial Protein Levels and Bioenergetics in the Musculus Rectus Femoris of Wfs1-Deficient Mice. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211181
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211181
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر