Metallothionein-I + II Reduces Oxidative Damage and Apoptosis after Traumatic Spinal Cord Injury in Rats
المؤلفون المشاركون
Méndez-Armenta, Marisela
Nava-Ruíz, Concepción
Diaz-Ruiz, Araceli
Orozco-Suárez, Sandra
Rios, Camilo
Santander, Iván
Islas, Marcela
Barón-Flores, Verónica
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-05
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
After spinal cord injury (SCI), some self-destructive mechanisms start leading to irreversible neurological deficits.
It is known that oxidative stress and apoptosis play a major role in increasing damage after SCI.
Metallothioneins I and II (MT) are endogenous peptides with known antioxidant, neuroprotective capacities.
Taking advantage of those capacities, we administered exogenous MT to rats after SCI in order to evaluate the protective effects of MT on the production of reactive oxygen species (ROS) and lipid peroxidation (LP), as markers of oxidative stress.
The activities of caspases-9 and -3 and the number of annexin V and TUNEL-positive cells in the spinal cord tissue were also measured as markers of apoptosis.
Rats were subjected to either sham surgery or SCI and received vehicle or two doses of MT (10 μg per rat) at 2 and 8 h after surgical procedure.
The results showed a significant increase in levels of MT protein by effect of SCI and SCI plus treatment at 12 h, while at 24 h an increase of MT was observed only in the injury plus treatment group (p<0.05).
ROS production was decreased by effect of MT in lesioned tissue; likewise, we observed diminished LP levels by MT effect both in the sham group and in the group with SCI.
Also, the results showed an increase in the activity of caspase-9 due to SCI, without changes by effect of MT, as compared to the sham group.
Caspase-3 activity was increased by SCI, and again, MT treatment reduced this effect only at 24 h after injury.
Finally, the results of the number of cells positive to annexin V and TUNEL showed a reduction due to MT treatment both at 24 and 72 h after the injury.
With the findings of this work, we conclude that exogenously administered MT has antioxidant and antiapoptotic effects after SCI.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rios, Camilo& Santander, Iván& Méndez-Armenta, Marisela& Nava-Ruíz, Concepción& Orozco-Suárez, Sandra& Islas, Marcela…[et al.]. 2018. Metallothionein-I + II Reduces Oxidative Damage and Apoptosis after Traumatic Spinal Cord Injury in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1211201
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rios, Camilo…[et al.]. Metallothionein-I + II Reduces Oxidative Damage and Apoptosis after Traumatic Spinal Cord Injury in Rats. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1211201
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rios, Camilo& Santander, Iván& Méndez-Armenta, Marisela& Nava-Ruíz, Concepción& Orozco-Suárez, Sandra& Islas, Marcela…[et al.]. Metallothionein-I + II Reduces Oxidative Damage and Apoptosis after Traumatic Spinal Cord Injury in Rats. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1211201
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211201
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر