Protective Effects of Lycium barbarum Extracts on UVB-Induced Damage in Human Retinal Pigment Epithelial Cells Accompanied by Attenuating ROS and DNA Damage
المؤلفون المشاركون
Chiu, Chien-Chih
Wu, Chang-Yi
Liu, Wangta
Hsieh, Feng-Chi
Hung, Chun-Tzu
Cheng, Kai-Chun
Chen, Yen-Chun
Wu, Yu-Jen
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-07
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The medicinal herb Lycium barbarum fruit has been widely used for improving and maintaining the health of the eyes in the Far East for many centuries.
This study is aimed at investigating whether protective effects generated from the aqueous (LBA) and ethanol (LBE) extracts of the L.
barbarum fruit existed against oxidative stress-induced apoptosis in human retinal pigment epithelial cells.
L.
barbarum extracts LBA and LBE exerted the activity of ROS scavenging and rescued UVB irradiation-induced growth inhibition in retinal pigment epithelial ARPE-19 cells.
Compared to LBA, the ethanol extract LBE exerted a superior protective activity on UVB-induced growth arrest in ARPE-19 cells.
Both L.
barbarum extracts significantly reduced cell cycle G2-arrest population in ARPE-19 cells.
Furthermore, the cytometer-based Annexin V/propidium iodide staining assay further showed that both L.
barbarum extracts protected ARPE-19 cells from UVB-induced apoptosis.
L.
barbarum extracts also reduced the activation of γH2AX, a sensor of DNA damage in ARPE-19 cells in a dose-responsive manner.
By using Ingenuity Pathway Analysis (IPA), the bioinformatics revealed that the protective effects of both LBA and LBE extracts might be involved in three signaling pathways, especially the Toll-like receptor (TLR) pathway associated with cellular proliferation.
Our study suggests that both ethanol and aqueous extracts of L.
barbarum exhibit antioxidant activity and rescue UVB-induced apoptosis of ARPE-19 cells.
Collectively, the ethanol extract exerts a superior effect on rescuing UVB-induced growth arrest of ARPE-19 compared to the aqueous extract, which might be associated with the activation of TLR signaling.
Our present work will benefit the preventive strategy of herbal medicine-based vision protection for treating eye diseases such as age-related macular degeneration in the future.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hsieh, Feng-Chi& Hung, Chun-Tzu& Cheng, Kai-Chun& Wu, Chang-Yi& Chen, Yen-Chun& Wu, Yu-Jen…[et al.]. 2018. Protective Effects of Lycium barbarum Extracts on UVB-Induced Damage in Human Retinal Pigment Epithelial Cells Accompanied by Attenuating ROS and DNA Damage. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211495
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hsieh, Feng-Chi…[et al.]. Protective Effects of Lycium barbarum Extracts on UVB-Induced Damage in Human Retinal Pigment Epithelial Cells Accompanied by Attenuating ROS and DNA Damage. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211495
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hsieh, Feng-Chi& Hung, Chun-Tzu& Cheng, Kai-Chun& Wu, Chang-Yi& Chen, Yen-Chun& Wu, Yu-Jen…[et al.]. Protective Effects of Lycium barbarum Extracts on UVB-Induced Damage in Human Retinal Pigment Epithelial Cells Accompanied by Attenuating ROS and DNA Damage. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211495
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211495
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر