Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle
المؤلفون المشاركون
Maiorka, Paulo
Hamilton, Thais Rose dos Santos
Mendes, Camilla Mota
Assis, Patrícia Monken de
Siqueira, Adriano Felipe Perez
Delgado, Juliana de Carvalho
Goissis, Marcelo Demarchi
Nichi, Marcílio
Visintin, José Antonio
Assumpção, Mayra Elena Ortiz D’Ávila
de Castro, Letícia S.
Mesquita, Leonardo Pereira
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-25
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Sperm DNA fragmentation is considered one of the main causes of male infertility.
The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis.
Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress.
We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis.
We observed higher percentages of ejaculated sperm with increased chromatin fragmentation in the heat stress group; a fact that was unexpectedly not observed in epididymal sperm.
Heat stress group presented a higher percentage of spermatozoa with DNA fragmentation and increased number of mRNA copies of transitional protein 1.
Epididymal sperm presented greater gene expression of protamine 1 on the 30th day of the spermatic cycle; however, no differences in protamine protein levels were observed in ejaculated sperm and testis.
Localization of proapoptotic protein BAX or BCL2 in testis was not different.
In conclusion, testicular heat stress increases ram sperm DNA fragmentation without changes in protamination and apoptotic patterns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hamilton, Thais Rose dos Santos& Siqueira, Adriano Felipe Perez& de Castro, Letícia S.& Mendes, Camilla Mota& Delgado, Juliana de Carvalho& Assis, Patrícia Monken de…[et al.]. 2018. Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1211628
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hamilton, Thais Rose dos Santos…[et al.]. Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1211628
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hamilton, Thais Rose dos Santos& Siqueira, Adriano Felipe Perez& de Castro, Letícia S.& Mendes, Camilla Mota& Delgado, Juliana de Carvalho& Assis, Patrícia Monken de…[et al.]. Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1211628
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211628
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر