RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells
المؤلفون المشاركون
Sato, Takuma
Takino, Jun-ichi
Nagamine, Kentaro
Nishio, Kazuto
Hori, Takamitsu
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
We have identified ras guanyl releasing protein 2 (rasgrp2) as a blood vessel related gene from Xenopus embryo.
In addition, we reported that RASGRP2 is also expressed in human umbilical vein endothelial cells (HUVEC).
It is known that RASGRP2 activates Ras-related protein 1 (Rap1).
However, the function of RASGRP2 in human vascular endothelium remains unknown.
Therefore, we performed functional analysis of RASGRP2 using immortalized HUVEC (TERT HUVEC).
We established a stable RASGRP2 overexpressing cell line (TERT HUVEC R) and mock cell line (mock).
Furthermore, we compared the activity of Rap1 and the generation of intracellular reactive oxygen species (ROS), which is related to cell death, in both cell lines.
Significant increase in Rap1 activity was observed in the TERT HUVEC R compared to the mock.
Furthermore, apoptosis by tumor necrosis factor-α (TNF-α) stimulation was significantly more reduced in the TERT HUVEC R than in the mock.
In the mock, apoptosis induced by TNF-α stimulation was decreased by pretreatment with diphenyleneiodonium (DPI), which is an inhibitor of NADPH oxidase (NOX).
However, in the TERT HUVEC R, apoptosis induced by TNF-α stimulation was not reduced after pretreatment of DPI.
Furthermore, there was no reduction in ROS production in the TERT HUVEC R after DPI pretreatment.
In addition, the difference in the degree of apoptosis induced by TNF-α stimulation in both cell lines was consistent with the difference in ROS production in the cell lines.
From these results, it was suggested that RASGRP2 activates Rap1 and the activated Rap1 suppresses apoptosis via NOX inhibition.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sato, Takuma& Takino, Jun-ichi& Nagamine, Kentaro& Nishio, Kazuto& Hori, Takamitsu. 2019. RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells. The Scientific World Journal،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1211839
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sato, Takuma…[et al.]. RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells. The Scientific World Journal No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1211839
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sato, Takuma& Takino, Jun-ichi& Nagamine, Kentaro& Nishio, Kazuto& Hori, Takamitsu. RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells. The Scientific World Journal. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1211839
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211839
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر