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Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells
المؤلفون المشاركون
Inkielewicz-Stepniak, Iwona
Barcińska, Ewelina
Wierzbicka, Justyna
Zauszkiewicz-Pawlak, Agata
Jacewicz, Dagmara
Dabrowska, Aleksandra
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-16
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Pancreatic ductal adenocarcinoma is one of the most aggressive human malignancies, where the 5-year survival rate is less than 4% worldwide.
Successful treatment of pancreatic cancer is a challenge for today’s oncology.
Several studies showed that increased levels of oxidative stress may cause cancer cells damage and death.
Therefore, we hypothesized that oxidative as well as nitro-oxidative stress is one of the mechanisms inducing pancreatic cancer programmed cell death.
We decided to use silver nanoparticles (AgNPs) (2.6 and 18 nm) as a key factor triggering the reactive oxygen species (ROS) and reactive nitrogen species (RNS) in pancreatic ductal adenocarcinoma cells (PANC-1).
Previously, we have found that AgNPs induced PANC-1 cells death.
Furthermore, it is known that AgNPs may induce an accumulation of ROS and alteration of antioxidant systems in different type of tumors, and they are indicated as promising agents for cancer therapy.
Then, the aim of our study was to evaluate the implication of oxidative and nitro-oxidative stress in this cytotoxic effect of AgNPs against PANC-1 cells.
We determined AgNP-induced increase of ROS level in PANC-1 cells and pancreatic noncancer cell (hTERT-HPNE) for comparison purposes.
We found that the increase was lower in noncancer cells.
Reduction of mitochondrial membrane potential and changes in the cell cycle were also observed.
Additionally, we determined the increase in RNS level: nitric oxide (NO) and nitric dioxide (NO2) in PANC-1 cells, together with increase in family of nitric oxide synthases (iNOS, eNOS, and nNOS) at protein and mRNA level.
Disturbance of antioxidant enzymes: superoxide dismutase (SOD1, SOD2, and SOD3), glutathione peroxidase (GPX-4) and catalase (CAT) were proved at protein and mRNA level.
Moreover, we showed cells ultrastructural changes, characteristic for oxidative damage.
Summarizing, oxidative and nitro-oxidative stress and mitochondrial disruption are implicated in AgNPs-mediated death in human pancreatic ductal adenocarcinoma cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Barcińska, Ewelina& Wierzbicka, Justyna& Zauszkiewicz-Pawlak, Agata& Jacewicz, Dagmara& Dabrowska, Aleksandra& Inkielewicz-Stepniak, Iwona. 2018. Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1212179
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Barcińska, Ewelina…[et al.]. Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1212179
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Barcińska, Ewelina& Wierzbicka, Justyna& Zauszkiewicz-Pawlak, Agata& Jacewicz, Dagmara& Dabrowska, Aleksandra& Inkielewicz-Stepniak, Iwona. Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1212179
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1212179
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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