Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc ExpressionACTH Secretion in AtT20 Cells
المؤلفون المشاركون
Sugawara, Akira
Yokoyama, Atsushi
Parvin, Rehana
Noro, Erika
Saito-Hakoda, Akiko
Shimada, Hiroki
Suzuki, Susumu
Shimizu, Kyoko
Miyachi, Hiroyuki
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-23
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Although therapeutic effects of the peroxisome proliferator-activated receptor gamma (PPAR-γ) agonists rosiglitazone and pioglitazone against Cushing’s disease have been reported, their effects are still controversial and inconsistent.
We therefore examined the effects of a novel PPAR-γ agonist, MEKT1, on Pomc expression/ACTH secretion using murine corticotroph-derived AtT20 cells and compared its effects with those of rosiglitazone and pioglitazone.
AtT20 cells were treated with either 1 nM~10 μM MEKT1, rosiglitazone, or pioglitazone for 24 hours.
Thereafter, their effects on proopiomelanocortin gene (Pomc) mRNA expression were studied by qPCR and the Pomc promoter (−703/+58) activity was demonstrated by luciferase assay.
Pomc mRNA expression and promoter activity were significantly inhibited by MEKT1 at 10 μM compared to rosiglitazone and pioglitazone.
SiRNA-mediated PPAR-γ knockdown significantly abrogated MEKT1-mediated Pomc mRNA suppression.
ACTH secretion from AtT20 cells was also significantly inhibited by MEKT1.
Deletion/point mutant analyses of Pomc promoter indicated that the MEKT1-mediated suppression was mediated via NurRE, TpitRE, and NBRE at −404/-383, −316/-309, and −69/-63, respectively.
Moreover, MEKT1 significantly suppressed Nur77, Nurr1, and Tpit mRNA expression.
MEKT1 also was demonstrated to inhibit the protein-DNA interaction of Nur77/Nurr1-NurRE, Tpit-TpitRE, and Nur77-NBRE by ChIP assay.
Taken together, it is suggested that MEKT1 could be a novel therapeutic medication for Cushing’s disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Parvin, Rehana& Noro, Erika& Saito-Hakoda, Akiko& Shimada, Hiroki& Suzuki, Susumu& Shimizu, Kyoko…[et al.]. 2018. Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc ExpressionACTH Secretion in AtT20 Cells. PPAR Research،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1212519
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Parvin, Rehana…[et al.]. Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc ExpressionACTH Secretion in AtT20 Cells. PPAR Research No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1212519
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Parvin, Rehana& Noro, Erika& Saito-Hakoda, Akiko& Shimada, Hiroki& Suzuki, Susumu& Shimizu, Kyoko…[et al.]. Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc ExpressionACTH Secretion in AtT20 Cells. PPAR Research. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1212519
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1212519
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر