Low-Concentration OxygenOzone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMPcGMP-NF-κBp65 Signaling in Chronic Radiculitis Rats
المؤلفون المشاركون
Fu, Zhi-Jian
Wang, Junnan
Wu, Mingyi
Lin, Xiaowen
Li, Yun
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
Oxygen/ozone therapy is a minimally invasive technique for the treatment of radiculitis from lumbar disc herniation.
This study aimed at investigating whether intrathecal administration of low-concentration oxygen/ozone could attenuate chronic radiculitis and mechanical allodynia after noncompressive lumbar disc herniation and at elucidating the underlying mechanisms.
Methods.
First, we transplanted autologous nucleus pulposus into dorsal root ganglions to establish chronic radiculitis in rats.
Then, filtered oxygen or oxygen/ozone (10, 20, or 30 μg/mL) was intrathecally injected on day 1 after surgery.
The ipsilateral paw withdrawal thresholds (PWTs) to mechanical stimuli were tested daily with von Frey filaments.
The expression of the tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), phosphodiesterase 2A (PDE2A), and nuclear factor- (NF-) κB/p65 in spinal dorsal horns was measured by enzyme-linked immunosorbent assay, polymerase chain reaction, and western blot on day 7 after surgery.
Results.
Chronic radiculitis was established in rats.
Intrathecal administration of 10 μg/mL, 20 μg/mL, or 30 μg/mL oxygen/ozone significantly attenuated the decreased mechanical PWTs, downregulated the overexpression of spinal TNF-α, IL-1β, and IL-6, and increased the expression of cGMP and cAMP in chronic radiculitis rats.
In addition, the effects of treatment with 20 μg/mL oxygen/ozone were greater than the effects of the 10 μg/mL or 30 μg/mL doses.
Moreover, intrathecal administration of 20 μg/mL oxygen/ozone reversed the increased levels of spinal PDE2A and NF-κB/p65 mRNA and protein expressions in rats with chronic radiculitis.
Conclusion.
Intrathecal administration of low-concentration oxygen/ozone alleviated mechanical allodynia and attenuated radiculitis, likely by a PDE2A-cGMP/cAMP-NF-κB/p65 signaling pathway in chronic radiculitis rats.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Junnan& Wu, Mingyi& Lin, Xiaowen& Li, Yun& Fu, Zhi-Jian. 2018. Low-Concentration OxygenOzone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMPcGMP-NF-κBp65 Signaling in Chronic Radiculitis Rats. Pain Research and Management،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1212574
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Junnan…[et al.]. Low-Concentration OxygenOzone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMPcGMP-NF-κBp65 Signaling in Chronic Radiculitis Rats. Pain Research and Management No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1212574
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Junnan& Wu, Mingyi& Lin, Xiaowen& Li, Yun& Fu, Zhi-Jian. Low-Concentration OxygenOzone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMPcGMP-NF-κBp65 Signaling in Chronic Radiculitis Rats. Pain Research and Management. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1212574
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1212574
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر