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Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity
المؤلفون المشاركون
Aldahmash, Abdullah
Alajez, Nehad M.
Manikandan, Muthurangan
Alfayez, Musaad
Ali, Dalia
Chalisserry, Elna P.
Hamam, Rimi
Kassem, Moustapha
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-14
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Bone marrow mesenchymal stem cells (BMSCs) are adult multipotent stem cells that can differentiate into mesodermal lineage cells, including adipocytes and osteoblasts.
However, the epigenetic mechanisms governing the lineage-specific commitment of BMSCs into adipocytes or osteoblasts are under investigation.
Herein, we investigated the epigenetic effect of romidepsin, a small molecule dual inhibitor targeting HDAC1 and HDAC2 identified through an epigenetic library functional screen.
BMSCs exposed to romidepsin (5 nM) exhibited enhanced adipocytic and osteoblastic differentiation.
Global gene expression and signaling pathway analyses of differentially expressed genes revealed a strong enrichment of genes involved in adipogenesis and osteogenesis in romidepsin-treated BMSCs during induction into adipocytes or osteoblasts, respectively.
Pharmacological inhibition of FAK signaling during adipogenesis or inhibition of FAK or TGFβ signaling during osteogenesis diminished the biological effects of romidepsin on BMSCs.
The results of chromatin immunoprecipitation combined with quantitative polymerase chain reaction indicated a significant increase in H3K9Ac epigenetic markers in the promoter regions of peroxisome proliferator-activated receptor gamma (PPARγ) and KLF15 (related to adipogenesis) or SP7 (Osterix) and alkaline phosphatase (ALP) (related to osteogenesis) in romidepsin-treated BMSCs.
Our data indicated that romidepsin is a novel in vitro modulator of adipocytic and osteoblastic differentiation of BMSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ali, Dalia& Chalisserry, Elna P.& Manikandan, Muthurangan& Hamam, Rimi& Alfayez, Musaad& Kassem, Moustapha…[et al.]. 2018. Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity. Stem Cells International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213289
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ali, Dalia…[et al.]. Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity. Stem Cells International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1213289
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ali, Dalia& Chalisserry, Elna P.& Manikandan, Muthurangan& Hamam, Rimi& Alfayez, Musaad& Kassem, Moustapha…[et al.]. Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213289
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213289
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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