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Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice
المؤلفون المشاركون
Levy, Bernard I
Kubis, Nathalie
Hilal, Rose
Poittevin, Marine
Pasteur-Rousseau, Adrien
Cogo, Adrien
Mangin, Gabrielle
Chevauché, Marie
Ziat, Yasmine
Vilar, José
Launay, Jean-Marie
Gautier, Jean-François
Broquères-You, Dong
Merkulova-Rainon, Tatyana
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-15
دولة النشر
مصر
عدد الصفحات
17
الملخص EN
Clinical trials of cell therapy in stroke favor autologous cell transplantation.
To date, feasibility studies have used bone marrow-derived mononuclear cells, but harvesting bone marrow cells is invasive thus complicating bedside treatment.
We investigated the therapeutic potential of peripheral blood-derived mononuclear cells (PB-MNC) harvested from diabetic patients and stimulated by ephrin-B2 (PB-MNC+) (500,000 cells), injected intravenously 18–24 hours after induced cerebral ischemia in mice.
Infarct volume, neurological deficit, neurogenesis, angiogenesis, and inflammation were investigated as were the potential mechanisms of PB-MNC+ cells in poststroke neurorepair.
At D3, infarct volume was reduced by 60% and 49% compared to unstimulated PB-MNC and PBS-treated mice, respectively.
Compared to PBS, injection of PB-MNC+ increased cell proliferation in the peri-infarct area and the subventricular zone, decreased microglia/macrophage cell density, and upregulated TGF-β expression.
At D14, microvessel density was decreased and functional recovery was enhanced compared to PBS-treated mice, whereas plasma levels of BDNF, a major regulator of neuroplasticity, were increased in mice treated with PB-MNC+ compared to the other two groups.
Cell transcriptional analysis showed that ephrin-B2 induced phenotype switching of PB-MNC by upregulating genes controlling cell proliferation, inflammation, and angiogenesis, as confirmed by adhesion and Matrigel assays.
Conclusions.
This feasibility study suggests that PB-MNC+ transplantation poststroke could be a promising approach but warrants further investigation.
If confirmed, this rapid, noninvasive bedside cell therapy strategy could be applied to stroke patients at the acute phase.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hilal, Rose& Poittevin, Marine& Pasteur-Rousseau, Adrien& Cogo, Adrien& Mangin, Gabrielle& Chevauché, Marie…[et al.]. 2018. Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice. Stem Cells International،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1213293
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hilal, Rose…[et al.]. Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice. Stem Cells International No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1213293
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hilal, Rose& Poittevin, Marine& Pasteur-Rousseau, Adrien& Cogo, Adrien& Mangin, Gabrielle& Chevauché, Marie…[et al.]. Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1213293
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213293
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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