A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications
المؤلفون المشاركون
Giannì, Aldo B.
Brini, Anna Teresa
Sordi, Valeria
Tremolada, Carlo
Pessina, Augusto
Alessandri, Giulio
Coccè, Valentina
Berenzi, Angiola
Versari, Silvia
Bosetto, Antonio
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-20
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures.
To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices.
Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment.
The aim of this study was to assess the presence of mesenchymal stromal cells in the drain bag of the device by using a prototype Lipogems processor to wash the lipoaspirate in standardized condition.
We found that, besides oil and blood residues, the drain bag contained single isolated cells easy to expand and with the typical characteristics of mesenchymal stromal cells that can be loaded with paclitaxel to use for drug-delivery application.
Our findings suggest the possibility to replace the drain bag with a “cell culture chamber” obtaining a new integrated device that, without enzymatic treatment, can isolate and expand mesenchymal stromal cells in one step with high good manufacturing practices compliance.
This system could be used to obtain mesenchymal stromal cells for regenerative purposes and for drug delivery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Coccè, Valentina& Brini, Anna Teresa& Giannì, Aldo B.& Sordi, Valeria& Berenzi, Angiola& Alessandri, Giulio…[et al.]. 2018. A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213373
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Coccè, Valentina…[et al.]. A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213373
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Coccè, Valentina& Brini, Anna Teresa& Giannì, Aldo B.& Sordi, Valeria& Berenzi, Angiola& Alessandri, Giulio…[et al.]. A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213373
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213373
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر