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Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5aβ-Catenin Signaling Pathway
المؤلفون المشاركون
Il Oh, Won
Kim, Miyeon
Kwon, Ji Hye
Bae, Yun Kyung
Kim, Gee-Hye
Choi, Soo Jin
Jin, Hye Jin
Yang, Yoon Sun
Jeon, Hong Bae
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-11
دولة النشر
مصر
عدد الصفحات
17
الملخص EN
Mesenchymal stem cells- (MSCs-) based therapies show different degrees of efficacies for the treatment of various diseases, including lipogenesis.
We evaluated the adipogenic differentiation ability of human umbilical cord blood-derived MSCs (hUCB-MSCs) from different donors and examined the contribution of the intracellular calcium (Ca2+) level to this diversity.
hUCB-MSCs treated with Ca2+ or the Ca2+ chelator BAPTA-AM increased and decreased adipogenic differentiation, respectively.
Canonical Wnt5a/β-catenin expression decreased during adipogenic differentiation of hUCB-MSCs.
Treatment with Wnt5a blocked the adipogenic differentiation of hUCB-MSCs and activated the Wnt pathway, with a decrease in the adipogenesis markers PPARγ and leptin, and reduced lipid vacuole-associated Oil red O activity.
In contrast, inhibition of the Wnt pathway with dickkopf-1 and β-catenin small interfering RNA transfection promoted the adipogenic potential of hUCB-MSCs.
Interestingly, the Ca2+-based system exhibited a synergic effect on adipogenic potential through the Wnt5a/β-catenin pathway.
Our data suggest that the variable adipogenic differentiation potential of hUCB-MSCs from different lots is due to variation in the intracellular Ca2+ level, which can be used as a marker to predict hUCB-MSCs selection for lipogenesis therapy.
Overall, these results demonstrate that exogenous calcium treatment enhanced the adipogenic differentiation of hUCB-MSCs via negatively regulating the Wnt5a/β-catenin signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bae, Yun Kyung& Kwon, Ji Hye& Kim, Miyeon& Kim, Gee-Hye& Choi, Soo Jin& Il Oh, Won…[et al.]. 2018. Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5aβ-Catenin Signaling Pathway. Stem Cells International،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1213522
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bae, Yun Kyung…[et al.]. Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5aβ-Catenin Signaling Pathway. Stem Cells International No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1213522
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bae, Yun Kyung& Kwon, Ji Hye& Kim, Miyeon& Kim, Gee-Hye& Choi, Soo Jin& Il Oh, Won…[et al.]. Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5aβ-Catenin Signaling Pathway. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1213522
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213522
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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