Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells
المؤلفون المشاركون
Wang, Huihui
Xu, Yuanyuan
Pi, Jingbo
Wu, Xiafang
Hu, Yuxin
Lou, Bin
Wu, Ruirui
Gao, Lanyue
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-11
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
In vitro culture of mesenchymal stem cells (MSCs) from mouse bone marrow (BM) has been hampered because of the low yield of MSCs during isolation and the contamination of hematopoietic cells during expansion.
The lack of specific mouse BM-MSC markers increases the difficulty.
Several techniques have been reported to improve the purity and in vitro growth of mouse BM-MSCs.
However, systematic report on comparison of characteristics in primary BM-MSCs between different culture conditions is rare.
Here, we studied the effects of oxygen concentrations and initial medium replacement intervals, along with cell passages, on mouse BM-MSCs isolated with differential adhesion method.
BM-MSCs exhibited elevated proliferative and clonogenic abilities in 5% oxygen compared with 10% and 21% oxygen, as well as a better expression of the MSC marker Sca-1.
Adipogenic and osteogenetic differentiation of BM-MSCs can be observed in both 21% and 5% oxygen.
Adipogenic differentiation appeared stronger under normoxia conditions.
BM-MSCs showed increased proliferative capacity and adipogenic/osteogenetic differentiation potential when initial medium replacement interval was 4 days compared with 1 day.
As passage number increased, cells were more MSC-like in morphology and in expression of surface markers (positive for CD29, CD44, and Sca-1 and negative for CD11b, CD19, and CD45).
These data provide new insight into optimizing the culture method and understanding the biological characteristics of mouse BM-MSCs during in vitro expansion.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Yuxin& Lou, Bin& Wu, Xiafang& Wu, Ruirui& Wang, Huihui& Gao, Lanyue…[et al.]. 2018. Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213526
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Yuxin…[et al.]. Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213526
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Yuxin& Lou, Bin& Wu, Xiafang& Wu, Ruirui& Wang, Huihui& Gao, Lanyue…[et al.]. Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213526
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213526
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر