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Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study
المؤلفون المشاركون
Waddington, Rachel J.
Sloan, Alastair J.
Alraies, Amr
Yusop, Norhayati
Battersby, Paul
Moseley, Ryan
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-22
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs).
This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation.
CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs.
Both populations exhibited consistent telomere lengths (12–17 kb), minimal senescence, and positive telomerase expression.
CB-MSCs (PD15) had significantly lower CFEs than PD50.
CB-MSCs and BM-MSCs both expressed MSC (CD73/CD90/CD105); embryonic (Nanog) and osteogenic markers (Runx2, osteocalcin) but no hematopoietic markers (CD45).
CB-MSCs (PD15) strongly expressed Oct4 and p16INK4A.
At early PDs, CB-MSCs possessed a strong osteogenic potency and low potency for adipogenesis, whilst BM-MSCs possessed greater overall bipotentiality for osteogenesis and adipogenesis.
At PD50, CB-MSCs demonstrated reduced potency for both osteogenesis and adipogenesis, compared to BM-MSCs at equivalent PDs.
This study demonstrates similarities in proliferative and mesenchymal cell characteristics between CB-MSCs and BM-MSCs, but contrasting multipotentiality.
Such findings support further comparisons of human CB-MSCs and BM-MSCs, facilitating selection of optimal MSC populations for regenerative medicine purposes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yusop, Norhayati& Battersby, Paul& Alraies, Amr& Sloan, Alastair J.& Moseley, Ryan& Waddington, Rachel J.. 2018. Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213528
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yusop, Norhayati…[et al.]. Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213528
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yusop, Norhayati& Battersby, Paul& Alraies, Amr& Sloan, Alastair J.& Moseley, Ryan& Waddington, Rachel J.. Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213528
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213528
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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