TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton
المؤلفون المشاركون
Atteya, Muhammad
Alajez, Nehad M.
Elsafadi, Mona
Manikandan, Muthurangan
Hashmi, Jamil Amjad
Iqbal, Zafar
Mubarak, Mohammed
Almalki, Sami
Shaheen, Sameerah
Alfayez, Musaad
Dawud, Raed Abu
Mahmood, Amer
Kassem, Moustapha
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-15
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
TGFβ is a potent regulator of several biological functions in many cell types, but its role in the differentiation of human bone marrow-derived skeletal stem cells (hMSCs) is currently poorly understood.
In the present study, we demonstrate that a single dose of TGFβ1 prior to induction of osteogenic or adipogenic differentiation results in increased mineralized matrix or increased numbers of lipid-filled mature adipocytes, respectively.
To identify the mechanisms underlying this TGFβ-mediated enhancement of lineage commitment, we compared the gene expression profiles of TGFβ1-treated hMSC cultures using DNA microarrays.
In total, 1932 genes were upregulated, and 1298 genes were downregulated.
Bioinformatics analysis revealed that TGFβl treatment was associated with an enrichment of genes in the skeletal and extracellular matrix categories and the regulation of the actin cytoskeleton.
To investigate further, we examined the actin cytoskeleton following treatment with TGFβ1 and/or cytochalasin D.
Interestingly, cytochalasin D treatment of hMSCs enhanced adipogenic differentiation but inhibited osteogenic differentiation.
Global gene expression profiling revealed a significant enrichment of pathways related to osteogenesis and adipogenesis and of genes regulated by both TGFβ1 and cytochalasin D.
Our study demonstrates that TGFβ1 enhances hMSC commitment to either the osteogenic or adipogenic lineages by reorganizing the actin cytoskeleton.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Elsafadi, Mona& Manikandan, Muthurangan& Almalki, Sami& Mubarak, Mohammed& Atteya, Muhammad& Iqbal, Zafar…[et al.]. 2018. TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213531
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Elsafadi, Mona…[et al.]. TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213531
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Elsafadi, Mona& Manikandan, Muthurangan& Almalki, Sami& Mubarak, Mohammed& Atteya, Muhammad& Iqbal, Zafar…[et al.]. TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213531
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213531
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر