Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation

المؤلفون المشاركون

Pang, Chi-Pui
Ng, Tsz Kin
Zhang, Mingzhi
Liang, Jiajian
Huang, Bing
Huang, Yuqiang
Chen, Chong-Bo

المصدر

Stem Cells International

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-10-16

دولة النشر

مصر

عدد الصفحات

13

الملخص EN

Retinal disease treatment by stem cell-based replacement relies on stem cell differentiation into retinal cells.

We previously demonstrated that human periodontal ligament-derived stem cells can be directed into retinal lineage upon induction.

Here, we report the transdifferentiation potential of human adipose-derived stem cells (ASCs) into retinal lineage and its enhancement by Notch signaling modulation.

Human ASCs, isolated from abdominal fat, expressed mesenchymal but not hematopoietic stem cell markers, and they can differentiate into adipocytes, chondrocytes, and osteoblasts in vitro.

Upon noggin/Dkk-1/IGF-1 induction, the treated ASCs showed elevated expression of retinal progenitor, retinal ganglion, and photoreceptor cell markers as well as the glutamate-evoked calcium response, which was not observed in the noninduced cells.

Compared to the regular induction treatment, Notch signaling activation by JAG1 enhanced the expression of retinal progenitor and precursor markers without affecting the glutamate-evoked calcium response.

In contrast, Notch signaling inhibition by DAPT showed more retinal ganglion cells, but delayed the response to glutamate stimulation.

In summary, our results revealed that human ASCs possess a retinal transdifferentiation potential upon noggin/Dkk-1/IGF-1 induction, which can further be enhanced by Notch signaling activation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Huang, Yuqiang& Ng, Tsz Kin& Chen, Chong-Bo& Huang, Bing& Liang, Jiajian& Pang, Chi-Pui…[et al.]. 2018. Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation. Stem Cells International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213650

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Huang, Yuqiang…[et al.]. Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation. Stem Cells International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1213650

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Huang, Yuqiang& Ng, Tsz Kin& Chen, Chong-Bo& Huang, Bing& Liang, Jiajian& Pang, Chi-Pui…[et al.]. Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213650

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1213650