AlginateHydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation
المؤلفون المشاركون
Sancilio, Silvia
Di Pietro, Roberta
Gallorini, Marialucia
Di Nisio, Chiara
Marsich, Eleonora
Schweikl, Helmut
Cataldi, Amelia
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-02
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Tissue engineering is widely recognized as a promising approach for bone repair and reconstruction.
Several attempts have been made to achieve materials that must be compatible, osteoconductive, and osteointegrative and have mechanical strength to provide a structural support.
Composite scaffolds consisting in biodegradable natural polymers are very promising constructs.
Hydroxyapatite (HAp) can support alginate as inorganic reinforcement and osteoconductive component of alginate/HAp composite scaffolds.
Therefore, HAp-strengthened polymer biocomposites offer a solid system to engineer synthetic bone substitutes.
In the present work, HAp was incorporated into an alginate solution and internal gelling was induced by addition of slowly acid-hydrolyzing D-gluconic acid delta-lactone for the direct release of calcium ions from HAp.
It has been previously demonstrated that alginate-based composites efficiently support adhesion of cancer bone cell lines.
Human dental pulp stem cells (DPSCs) identified in human dental pulp are clonogenic cells capable of differentiating in multiple lineage.
Thus, this study is aimed at verifying the mineralization and differentiation potential of human DPSCs seeded onto scaffolds based on alginate and nano-hydroxyapatite.
For this purpose, gene expression profile of early and late mineralization-related markers, extracellular matrix components, viability parameters, and oxidative stress occurrence were evaluated and analyzed.
In summary, our data show that DPSCs express osteogenic differentiation-related markers and promote calcium deposition and biomineralization when growing onto Alg/HAp scaffolds.
These findings confirm the use of Alg/HAp scaffolds as feasible composite materials in tissue engineering, being capable of promoting a specific and successful tissue regeneration as well as mineralized matrix deposition and sustaining natural bone regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sancilio, Silvia& Gallorini, Marialucia& Di Nisio, Chiara& Marsich, Eleonora& Di Pietro, Roberta& Schweikl, Helmut…[et al.]. 2018. AlginateHydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation. Stem Cells International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213718
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sancilio, Silvia…[et al.]. AlginateHydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation. Stem Cells International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1213718
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sancilio, Silvia& Gallorini, Marialucia& Di Nisio, Chiara& Marsich, Eleonora& Di Pietro, Roberta& Schweikl, Helmut…[et al.]. AlginateHydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213718
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213718
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر