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Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH
المؤلفون المشاركون
Shibulal, Biji
Al-Bahry, Saif N.
Al-Wahaibi, Yahya M.
Elshafie, Abdulkader E.
Al-Bemani, Ali S.
Joshi, Sanket J.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-21
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The identification of potential hydrocarbon utilizing bacteria is an essential requirement in microbial enhanced oil recovery (MEOR).
Molecular approaches like proteomic and genomic characterization of the isolates are replacing the traditional method of identification with systemic classification.
Genotypic profiling of the isolates includes fingerprint or pattern-based technique and sequence-based technique.
Understanding community structure and dynamics is essential for studying diversity profiles and is challenging in the case of microbial analysis.
The present study aims to understand the bacterial community composition from different heavy oil contaminated soil samples collected from geographically related oil well areas in Oman and to identify spore-forming hydrocarbon utilizing cultivable bacteria.
V4 region of 16S rDNA gene was the target for Ion PGM™.
A total of 825081 raw sequences were obtained from Ion torrent from all the 10 soil samples.
The species richness and evenness were found to be moderate in all the samples with four main phyla, Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria, the most abundant being Firmicutes.
Bacillus sp.
ubiquitously dominated in all samples followed by Paenibacillus, which was followed by Brevibacillus, Planococcus, and Flavobacterium.
Principal Coordinate Analysis (PCoA) and UPGMA dendrogram clustered the 10 soil samples into four main groups.
Weighted UniFrac significance test determined that there was significant difference in the communities present in soil samples examined.
It can be concluded that the microbial community was different in all the 10 soil samples with Bacillus and Paenibacillus sp.
as predominating genus.
The 16S rDNA sequencing of cultivable spore-forming bacteria identified the hydrocarbon utilizing bacteria as Bacillus and Paenibacillus sp.
and the nucleotide sequences were submitted to NCBI GenBank under accession numbers KP119097–KP119115.
Bacillus and Paenibacillus sp., which were relatively abundant in the oil fields, can be recommended to be chosen as candidates for hydrocarbon utilization study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shibulal, Biji& Al-Bahry, Saif N.& Al-Wahaibi, Yahya M.& Elshafie, Abdulkader E.& Al-Bemani, Ali S.& Joshi, Sanket J.. 2018. Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH. Scientifica،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213791
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shibulal, Biji…[et al.]. Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH. Scientifica No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213791
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shibulal, Biji& Al-Bahry, Saif N.& Al-Wahaibi, Yahya M.& Elshafie, Abdulkader E.& Al-Bemani, Ali S.& Joshi, Sanket J.. Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH. Scientifica. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213791
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213791
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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