Detection of viable but non-culturable (Vbnc) Escherichia coli from beef liver using Pma-PCR assay
المؤلفون المشاركون
Husayn, Diya al-Din E.
Ghayt, Amr Sad Shihatah
المصدر
Alexandria Journal of Veterinary Sciences
العدد
المجلد 69، العدد 2 (30 إبريل/نيسان 2021)، ص ص. 1-6، 6ص.
الناشر
جامعة الإسكندرية كلية الطب البيطري
تاريخ النشر
2021-04-30
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Escherichia coli, the most important foodborne pathogen worldwide, can go into a state of dormancy called viable but nonculturable (VBNC) state under various stress conditions.
Hence, it escapes detection by traditional culturing methods.
The current research made use of Propidium monoazide (PMA)-Polymerase chain reaction (PCR) assay in order to verify whether E.
coli, especially O157 strains, converted to the VBNC state under the influence of cool-warm and freeze-thaw stresses.
120 beef liver samples were collected from local markets.
The samples that were positive to pathogenic E.
coli were treated with cooling (4 °C)-warm and freezing (-18 °C)-thaw cycles to induce the VBNC state.
Samples that demonstrated the inability to form colonies on EMB agar were tested by PMA-PCR assays to confirm the existence of VBNC E.
coli.
9 samples (7.5 %) of 120 beef liver samples were positive to pathogenic E.
coli by microbiological tests.
Cool-warm and freeze-thaw stresses on the 9 samples led to the inability of 3 of them to grow on EMB agar plates.
By using PMA-PCR assay, we found that 4 °C cool-warm cycle induced E.
coli into VBNC state at a higher percentage ratio than -18 °C freeze-thaw cycle.
Even though further experiments are still required, in rare cases, at -18 °C, E.
coli VBNC may lose expression of genes responsible for viability and metabolic activity.
Additionally, our study evaluated the PMA-PCR assay as a rapid and accurate method to check for the presence of VBNC-associated foodborne E.
coli O157.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayn, Diya al-Din E.& Ghayt, Amr Sad Shihatah. 2021. Detection of viable but non-culturable (Vbnc) Escherichia coli from beef liver using Pma-PCR assay. Alexandria Journal of Veterinary Sciences،Vol. 69, no. 2, pp.1-6.
https://search.emarefa.net/detail/BIM-1252670
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayn, Diya al-Din E.& Ghayt, Amr Sad Shihatah. Detection of viable but non-culturable (Vbnc) Escherichia coli from beef liver using Pma-PCR assay. Alexandria Journal of Veterinary Sciences Vol. 69, no. 2 (Apr. 2021), pp.1-6.
https://search.emarefa.net/detail/BIM-1252670
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayn, Diya al-Din E.& Ghayt, Amr Sad Shihatah. Detection of viable but non-culturable (Vbnc) Escherichia coli from beef liver using Pma-PCR assay. Alexandria Journal of Veterinary Sciences. 2021. Vol. 69, no. 2, pp.1-6.
https://search.emarefa.net/detail/BIM-1252670
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5-6
رقم السجل
BIM-1252670
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر