Molecular detection of integron in Staphylococcus aureus isolated from ruminants
المؤلفون المشاركون
Ubayd, Sawsan Khamis Mahmud
Turki, Hilmi A.
Abd al-Rahman, Amr M.
المصدر
Alexandria Journal of Veterinary Sciences
العدد
المجلد 68، العدد 1 (31 يناير/كانون الثاني 2021)، ص ص. 90-96، 7ص.
الناشر
جامعة الإسكندرية كلية الطب البيطري
تاريخ النشر
2021-01-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Staphylococcus aureus is one of the most important pathogens of humans and animals, asymptomatically colonizes the anterior nares of humans and animals and it is considered one of the most significant etiological agents of intramammary infection in dairy ruminants, causing both clinical and subclinical mastitis, causing wound infection and dermatitis.
A total number of 164 samples (mastitic milk, nasal swabs, wound swabs, abscesses′ contents) were collected from ruminant animals (cattle, sheep and goat) either individually or from farms in Alexandria governorate and subjected to bacteriological examination.
Seventeen Staphylococcus aureus isolates were identified morphologically, biochemically.
Antibiogram profile of the isolates were carried out against 11 antimicrobials.
16/17 (94%) of isolates showed resistance to at least one antimicrobial, 11/17(64.7) of the isolates showed resistance to three or more of antimicrobials (multi-drug resistance), the highest sensitivity was observed for vancomycin and amoxicillin/clavulanic acid 17/17 (100%) while the highest resistance was observed for penicillin 14/17 (82.4%), and gentamicin 9/17(53%).
Eight out of seventeen isolates (47%) showed resistance to oxacillin/cefoxitin (methicillin resistant S.
aureus) all of them were multi drug resistant (MDR).
Ten of MDR S.
aureus isolates were molecularly screened for integron.
Class 1 integron cassette were detected in 1/10 of tested isolates which exposed to further sequence analysis revealing dfrA15 gene cassette which encodes trimethoprim resistance.
Conclusion: this study report, for the first time, the detection and identification of class 1 integron containing dfrA15 gene in S.
aureus isolates which were deposited in the GenBank as Staphylococcus aureus strain AR2020 dihydrofolate reductase 15 (dfrA15) gene with accession number MW036489
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Rahman, Amr M.& Turki, Hilmi A.& Ubayd, Sawsan Khamis Mahmud. 2021. Molecular detection of integron in Staphylococcus aureus isolated from ruminants. Alexandria Journal of Veterinary Sciences،Vol. 68, no. 1, pp.90-96.
https://search.emarefa.net/detail/BIM-1256596
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ubayd, Sawsan Khamis Mahmud…[et al.]. Molecular detection of integron in Staphylococcus aureus isolated from ruminants. Alexandria Journal of Veterinary Sciences Vol. 68, no. 1 (Jan. 2021), pp.90-96.
https://search.emarefa.net/detail/BIM-1256596
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Rahman, Amr M.& Turki, Hilmi A.& Ubayd, Sawsan Khamis Mahmud. Molecular detection of integron in Staphylococcus aureus isolated from ruminants. Alexandria Journal of Veterinary Sciences. 2021. Vol. 68, no. 1, pp.90-96.
https://search.emarefa.net/detail/BIM-1256596
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 95-96
رقم السجل
BIM-1256596
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر