Histopathological assessment of nano n-acetyl cysteine effect on postoperative adhesion in rats

الملخص EN

Abdominal adhesion is one of the main and important post-surgical problems and, according to available literature, the probability of their occurrence after surgery is higher than 50% .

The present study intended to estimate the effect of Nano n-acetylcysteine on macroscopic and microscopic features post-surgical adhesions in an animal model.

Twenty-five adult male Wistar rats, 200-220 gr, were randomly divided into five equal groups and underwent intramuscular anesthesia by injection of ketamine 10% at 50 mg/kg and injection of xylazine 2% at 5 mg/ kg.

The treatments included three Nano-NAC groups at different dosages 50, 75 and 150 mg/kg, an NAC group, and a control group.

On days 14 and 28 post-surgery, the abdomens of all rats were re-opened for macroscopic and microscopic evaluation of adhesions.

Based on the results of macroscopic evaluation, the group receiving Nano-NAC 50 mg/kg had the lowest and the control group the highest mean adhesion scores on days14 and 28 post-operations.

Histopathological evaluation also revealed that the group treated with Nano-NAC 50 mg/kg had the lowest level of inflammation and fibrosis score among the five groups on days 14 and 28 post-operation.

Considering the results obtained in our experimental model and the increasing use of nanotechnology in the manufacture of biomedical drugs, it seems that use of Nano-NAC at low doses 50 and 75mg/kg after laparotomy can significantly reduce adhesions.