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L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis
المؤلفون المشاركون
Reddy, Gopal
Shawqi, Sharif
Urabi, Sahar H.
Wirtu, Gemechu
المصدر
Alexandria Journal of Veterinary Sciences
العدد
المجلد 67، العدد 1 (31 أكتوبر/تشرين الأول 2020)، ص ص. 1-8، 8ص.
الناشر
جامعة الإسكندرية كلية الطب البيطري
تاريخ النشر
2020-10-31
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The objective of the current study was to assess the effect of L-Carnitine as a stimulant of lipid catabolism in vitro maturation (IVM) and in vitro culture (IVC) media on IVM rate (nuclear maturation) and embryonic development.
A total of 495 oocytes were assigned into two groups; the control group, oocytes matured in TCM-199 medium; L-Carnitine group oocytes matured in TCM-199 medium supplemented with L-Carnitine (0.5mg/ml).
After that 250 oocytes were stained with Hoechst 33342 then with Nile Red dye.
The nuclear maturation and oocyte lipid content were determined under a fluorescence microscope.
The other matured oocytes were parthenogentically activated using Thimerosal and DL- Dithiothreitol (DDT).
Presumptive zygotes were incubated in vitro culture 1 and 2, embryonic development was evaluated for morula and blastocyst stages.
The Metaphase II rates were 16.2% and 42.1% in the control group and L-carnitine group, respectively.
Overall, 9.3% and 14.9% of the oocytes reached at least the MI stage in the control group and L-carnitine group respectively.
The addition of L-carnitine in IVM media resulted in a significant decrease in the amount of lipid in cat oocyte.
Moreover, the incubation of matured oocytes in the L-carnitine group in IVC1 lead to significant increase in cleaved cells 29.4% in comparison with the control group 15.4%.
The incubation of cleaved cell of the L-carnitine group in IVC2 lead to decrease significantly in degenerated cells 12.5 % compared to the control group 42.9% while caused an increase significantly in morula 50% compared to the control group 33.3% and blastocyst 37.5% comparison with the control group 23.8%.
In conclusion, the use of L-Carnitine as a supplement in IVM media decrease lipid content of cat oocyte and subsequent enhance the nuclear and cytoplasmic maturation as demonstrated by increased parthenogentic development.-
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shawqi, Sharif& Urabi, Sahar H.& Reddy, Gopal& Wirtu, Gemechu. 2020. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. Alexandria Journal of Veterinary Sciences،Vol. 67, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-1256794
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shawqi, Sharif…[et al.]. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. Alexandria Journal of Veterinary Sciences Vol. 67, no. 1 (Oct. 2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1256794
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shawqi, Sharif& Urabi, Sahar H.& Reddy, Gopal& Wirtu, Gemechu. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. Alexandria Journal of Veterinary Sciences. 2020. Vol. 67, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-1256794
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 7-8
رقم السجل
BIM-1256794
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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