Polymer chain reaction (PCR) : principle and applications
المؤلف
المصدر
Ibn al-Haitham Journal for Pure and Applied Science
العدد
المجلد 34، العدد 4 (31 أغسطس/آب 2021)، ص ص. 35-44، 10ص.
الناشر
جامعة بغداد كلية التربية ابن الهيثم
تاريخ النشر
2021-08-31
دولة النشر
العراق
عدد الصفحات
10
التخصصات الرئيسية
الموضوعات
الملخص EN
The new, standard molecular biologic system for duplicating DNA enzymatically devoid of employing a living organism, like E.
coli or yeast, represents polymerases chain reaction (PCR).
This technology allows an exponential intensification of a minor quantity of DNA molecule several times.
Analysis can be straightforward with more DNA available.
A thermal heat cycler performs a polymerization chain reaction that involves repeated cycles of heating and cooling the reactant tubes at the desired temperature for each reaction step.
A heated deck is positioned on the upper reaction tube to avoid evaporating the reaction mixture (normally volumes range from 15 to 100 l per tube), or an oil layer can be placed on a reaction mixture surface.
The amplified DNA fragment is determined based on selecting primers in addition to the starting and end of the DNA fragment.
The primers stand for short, artificial DNA stripes, no higher than fifty (typically 18-25bp) nucleotides have been based on a starting and ending of DNA fragment to be amplified.
DNA-polymerase connects and starts a new DNA strand synthesisThe PCR products can be visualized by dual foremost methods: (1) staining of the product of DNA amplified by a chemical dye like bromide ethidium, or (2) marking of fluorescent dyes (fluorophores) PCR primers or nucleotides before amplification of PCRs.
PCR offers some benefits.
First, it is a simple method of understanding and using and quick results.
It has an extremely sensitive technology with the potential for sequencing, cloning, and analyzing millions or milliards of copies of a particular The new, standard molecular biologic system for duplicating DNA enzymatically devoid of employing a living organism, like E.
coli or yeast, represents polymerases chain reaction (PCR).
This technology allows an exponential intensification of a minor quantity of DNA molecule several times.
Analysis can be straightforward with more DNA available.
A thermal heat cycler performs a polymerization chain reaction that involves repeated cycles of heating and cooling the reactant tubes at the desired temperature for each reaction step.
A heated deck is positioned on the upper reaction tube to avoid evaporating the reaction mixture (normally volumes range from 15 to 100 l per tube), or an oil layer can be placed on a reaction mixture surface.
The amplified DNA fragment is determined based on selecting primers in addition to the starting and end of the DNA fragment.
The primers stand for short, artificial DNA stripes, no higher than fifty (typically 18-25bp) nucleotides have been based on a starting and ending of DNA fragment to be amplified.
DNA-polymerase connects and starts a new DNA strand synthesisThe PCR products can be visualized by dual foremost methods: (1) staining of the product of DNA amplified by a chemical dye like bromide ethidium, or (2) marking of fluorescent dyes (fluorophores) PCR primers or nucleotides before amplification of PCRs.
PCR offers some benefits.
First, it is a simple method of understanding and using and quick results.
It has an extremely sensitive technology with the potential for sequencing, cloning, and analyzing millions or milliards of copies of a particular product.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shafiq, Nurhan Khalid. 2021. Polymer chain reaction (PCR) : principle and applications. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 34, no. 4, pp.35-44.
https://search.emarefa.net/detail/BIM-1281380
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shafiq, Nurhan Khalid. Polymer chain reaction (PCR) : principle and applications. Ibn al-Haitham Journal for Pure and Applied Science Vol. 34, no. 4 (2021), pp.35-44.
https://search.emarefa.net/detail/BIM-1281380
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shafiq, Nurhan Khalid. Polymer chain reaction (PCR) : principle and applications. Ibn al-Haitham Journal for Pure and Applied Science. 2021. Vol. 34, no. 4, pp.35-44.
https://search.emarefa.net/detail/BIM-1281380
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 42-44
رقم السجل
BIM-1281380
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر