Isolation, purification and characterization of novel phytomitogen from nigella sativa seeds
المؤلف
المصدر
Engineering and Technology Journal
العدد
المجلد 38، العدد 1B (31 يناير/كانون الثاني 2020)، ص ص. 26-33، 8ص.
الناشر
تاريخ النشر
2020-01-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The objective of the present study was to extract and purified proliferative factor for peripheral blood lymphocytes from nigella sativa seeds.
dimeric 44, 32 and 26 -kDa glucosamine-specific lectins were extracted and purified from seeds of n.
sativa l.
by using different ionic strength solvents (deionized distilled water, phosphate buffer slain and 0.1 M HCL) purification procedure included, precipitation in saturated ammonium sulphate, then sephadex g-100 gel filtration was used for further purification, finally electrophoresis by polyacrylamide gel to estimate the molecular weight in the presence of sodium dodecylsulphate.
single absorbance peak at 280 nm was recorded in each extracted method.
the temperature and pH value on mitogenic activity was studied in temperature ranged from 4°c-60°c, the mitogenic activity reduced with gradual increase of temperatures, 50% of activity lost at temperature greater than 40°c, while the optimum pH for mitogenic activity ranged from 5-9, at pH value less than 3, and greater than 12 the activity disappeared.
interestingly, lectin extracted from n.
sativa seeds have high activity especially that extracted with 0.1 M HCL and showed stability at temperature from 4°c to 10°c and ph from 5 to 9.
the pure lectin is a homodimeric molecule of 26 kDa with subunit molecular mass of 12.9 kDa.
the novel lectin exceed the PHA-sigma in mitogenic activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Halim, Azhar M.. 2020. Isolation, purification and characterization of novel phytomitogen from nigella sativa seeds. Engineering and Technology Journal،Vol. 38, no. 1B, pp.26-33.
https://search.emarefa.net/detail/BIM-1283382
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Halim, Azhar M.. Isolation, purification and characterization of novel phytomitogen from nigella sativa seeds. Engineering and Technology Journal Vol. 38, no. 1B (2020), pp.26-33.
https://search.emarefa.net/detail/BIM-1283382
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Halim, Azhar M.. Isolation, purification and characterization of novel phytomitogen from nigella sativa seeds. Engineering and Technology Journal. 2020. Vol. 38, no. 1B, pp.26-33.
https://search.emarefa.net/detail/BIM-1283382
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 32-33
رقم السجل
BIM-1283382
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر