Evaluation of lentiviral genetic mutations
العناوين الأخرى
تطور الطفرات الوراثية للفيروسات البطيئة (Lentivirus)
المؤلفون المشاركون
Irahumah, al-Sadiq
Guiguen, François
Gauthier, Dominique
Chebloune, Yahya
المصدر
مجلة جامعة سبها للعلوم البحتة و التطبيقية
الناشر
تاريخ النشر
2020-12-31
دولة النشر
ليبيا
عدد الصفحات
7
التخصصات الرئيسية
الملخص الإنجليزي
The small ruminant lentiviruses (SRLV) have the potential to change genetically and switch from an host to another.
In this paper, we studied genetic evaluation that accompanied infestation and adaptation of SRLVs in hybrids.
Three blood samples, one year apart, were taken from Hybrids living in two sites in French Alps.
Antibodies to SRLV were sought in serum using a commercial ELISA to p28 antigen, and ficoll-isolated monocytes were cultured in macrophage differentiation medium to detect active viral infection.
DNA extracted from non-cultured monocytes was amplified by PCR and Nested PCR.
Amplicons were sequenced and analysed.
The Nested PCR technique specifically amplified a 513 nucleotide fragment of the gag gene including the 3' portion.
The sequences alignments shows two types of genetic mutations: deletion and replacement.
The sequences obtained from the samples taken in first year showed a diversity comparable to that observed in the reference virus.
After one year of cohabitation, the sequences of the virus showed very important genetic mutations including a deletion of 6 nucleotides.
Finally, after 3 years of cohabitation, the virus sequences included more genetic mutations.
The percentages of discrepancies between the proviral sequences obtained and that of reference virus were on average from 6.1 to 8.8% in nucleotides.
Compared with proviral sequences obtained in first year, the average percentages of divergence was 2.6% in nucleotides in second year and 2.9% in third year.
The average of the internal divergence percentages was 0.56 - 2.79%.
These differences were also shown by the phylogenetic tree.
نوع البيانات
أوراق مؤتمرات
رقم السجل
BIM-1285096
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Irahumah, al-Sadiq& Guiguen, François& Gauthier, Dominique& Chebloune, Yahya. 2020-12-31. Evaluation of lentiviral genetic mutations. . Vol. 19, no. 5 (2020), pp.159-165.Sabha Murzuq : Sabha University.
https://search.emarefa.net/detail/BIM-1285096
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Irahumah, al-Sadiq…[et al.]. Evaluation of lentiviral genetic mutations. . Sabha Murzuq : Sabha University. 2020-12-31.
https://search.emarefa.net/detail/BIM-1285096
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Irahumah, al-Sadiq& Guiguen, François& Gauthier, Dominique& Chebloune, Yahya. Evaluation of lentiviral genetic mutations. .
https://search.emarefa.net/detail/BIM-1285096
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر