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Laboratory microbiological and chemical analysis for detection of water pollution in fresh water fish farms
المؤلفون المشاركون
Khalifah, Amal Muhammad
al-Jamal, Ghadah Ahmad
al-Jawhari, Muhammad S.
Barakat, Muhammad E.
Fadl, Sabirin Izzat
المصدر
Alexandria Journal of Veterinary Sciences
العدد
المجلد 66، العدد 1 (31 يوليو/تموز 2020)، ص ص. 76-88، 13ص.
الناشر
جامعة الإسكندرية كلية الطب البيطري
تاريخ النشر
2020-07-31
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
This work carried out to analyze the microbiology and physicochemical parameters of water in some Nile tilapia fish farms in Kafrelsheikh Governorate.
Total bacterial and total coliform count were carried out, as well as the presence of E.faecalis, E.coli, and E.coli O157 in both fish and farms water.
The level of total bacterial count in water varied between 4.3x104 and 1.3x106 CFU/ml.
The most probable number (MPN) of total coliform ranged between <3 and >1100/ml between different sampling sites.
E.faecalis verified in 28% and 38% of fish, and, water samples respectively E.coli isolated in 18% and 34% from fish and water samples.
E.coli O157 verified in 2% of fish samples and does not present in water samples.
Sero-grouping of E.coli isolates revealed that E.coli belongs to five sero-groups (O6, O27, O125, O126, and O157).
Results of microbiology were confirmed by results of physicochemical parameters of the water samples where values of pH, conductivity, salinity, ammonia, nitrite, sulphate, chlorine, chloride, total solids, total suspended solids, and total dissolved solids in farms number two, six, and ten when compared with other farms were significantly (P<0.05) increased in the same farms, which contain high coliform count.
Multiple antibiotic resistance was recorded in 18/28 E.faecalis isolates (64.3%) and in all E.coli isolates 20/20 (100%).
Eight randomly selected isolates of E.faecalis were confirmed by polymerase chain reaction (PCR) using specific 16Sr RNA showed that seven from the eight examined isolates were positive with a product band (310 bp).
In addition, the two isolates of E.coli O157 were harboring rfbE gene with a product band (134 bp).
It could be concluded that, microbial and chemical evaluation of water in fish farms is the best witness for levels of water pollution and risk of usage of agriculture wastewater.
Besides the development of antimicrobial resistance by different bacteria calling for concern to public health hazard.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khalifah, Amal Muhammad& al-Jamal, Ghadah Ahmad& Fadl, Sabirin Izzat& al-Jawhari, Muhammad S.& Barakat, Muhammad E.. 2020. Laboratory microbiological and chemical analysis for detection of water pollution in fresh water fish farms. Alexandria Journal of Veterinary Sciences،Vol. 66, no. 1, pp.76-88.
https://search.emarefa.net/detail/BIM-1289229
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Jamal, Ghadah Ahmad…[et al.]. Laboratory microbiological and chemical analysis for detection of water pollution in fresh water fish farms. Alexandria Journal of Veterinary Sciences Vol. 66, no. 1 (Jul. 2020), pp.76-88.
https://search.emarefa.net/detail/BIM-1289229
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khalifah, Amal Muhammad& al-Jamal, Ghadah Ahmad& Fadl, Sabirin Izzat& al-Jawhari, Muhammad S.& Barakat, Muhammad E.. Laboratory microbiological and chemical analysis for detection of water pollution in fresh water fish farms. Alexandria Journal of Veterinary Sciences. 2020. Vol. 66, no. 1, pp.76-88.
https://search.emarefa.net/detail/BIM-1289229
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 85-88
رقم السجل
BIM-1289229
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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