Relationship GDF9B (BMP-15)‎ gene polymorphism with litter size in Iraqi Awassi sheep

الملخص EN

Thirty-two of ewes from Iraqi Awassi breed and thirty-eight of her births used in the present study with the same feeding conditions from Al-Kafeel sheep station, Karbala, molecular analysis were completed in the Molecular Genetics Laboratory at the College of Agriculture / University of Basrah.

We investigated fecundity genes-bone morphogenetic protein 15 (BMP-15) in order to detect any possible mutations in this gene in iraqi Awassi ewes using DNA sequencing technique.

We identified one single nucleotide polymorphism (SNP) from the studied fragment of gene BMP-15 which is 356bp at (T287C) site, the frequency polymorphisms of this site for CC, CT and TT were 0.59, 0.22 and 0.19, as for the frequency of allele A and B, it was 0.69 and 0.31.

The difference of the least squares means for litter size between polymorphisms of BMP-15 gene CC, CT and TT was not significant (p>0.05) with this study, the values of the litter size were recorded 1.16, 1.29 and 1.17 for polymorphism TT, CT and CC respectively.

The results did not show any significant effect (P<0.05) of the difference in the BMP-15 gene polymorphisms at site (T287C) on any of the growth traits as lamb weight at birth (BW), weaning (WW) and average daily gain (ADG).

The sequences of BMP-15 gene were submitted to the GenBank database, the Sequences were recorded under accession numbers LC495449 into Thirty-two of ewes from Iraqi Awassi breed and thirty-eight of her births used in the present study with the same feeding conditions from Al-Kafeel sheep station, Karbala, molecular analysis were completed in the Molecular Genetics Laboratory at the College of Agriculture / University of Basrah.

We investigated fecundity genes-bone morphogenetic protein 15 (BMP-15) in order to detect any possible mutations in this gene in iraqi Awassi ewes using DNA sequencing technique.

We identified one single nucleotide polymorphism (SNP) from the studied fragment of gene BMP-15 which is 356bp at (T287C) site, the frequency polymorphisms of this site for CC, CT and TT were 0.59, 0.22 and 0.19, as for the frequency of allele A and B, it was 0.69 and 0.31.

The difference of the least squares means for litter size between polymorphisms of BMP-15 gene CC, CT and TT was not significant (p>0.05) with this study, the values of the litter size were recorded 1.16, 1.29 and 1.17 for polymorphism TT, CT and CC respectively.

The results did not show any significant effect (P<0.05) of the difference in the BMP-15 gene polymorphisms at site (T287C) on any of the growth traits as lamb weight at birth (BW), weaning (WW) and average daily gain (ADG).

The sequences of BMP-15 gene were submitted to the GenBank database, the Sequences were recorded under accession numbers LC495449 into LC495469.