Effect of plant growth regulators and explant source on the induction of callus of dianthus caryophyllus L.

الملخص EN

In order to investigate the possibility for in in vitro fast regeneration ofDianthus caryophyllus, different concentrations of Kinetin (Kin), 2, 4-DichlorophenoxyAcetic Acid (2, 4-D) had been tested for induction of callus by leaves and stems explantusing Murashige and Skoog (MS) medium.

After two months in culture, response callusin the induction rate, fresh and dry weight, texture and color were evaluated.

MSmedium that contained 4.5 mg.

L-1 2, 4-D was suitable for callus induction with leafexplants.

The combination of 0.4 mg.

L-1 Kin and 2.0 mg.

L-1 2, 4-D also demonstrated awonderful induction of callus on stem explants.

In addition to the culture mediumcomplemented by Kin and 2, 4-D, the influence of Zeatin at various concentrations (0.0, 0.4, 0.8 or 1.0 mg.

L-1) was assessed.

In MS medium that contained 0.4 mg.

L-1 Kin+ 2.0mg.

L-1 2, 4-D+ 1.0 mg.

L-1 Zeatin, rapid callus induction and also more callus proliferationfrom stem explants were detected.

Results indicate on callus induction that, Zeatin wasmore successful than Kin or 2, 4-D, but other combinations directly developed a shoot.

Callus obtained from stems explant was friable, white and yellow, while callus from leafexplants was green.