Genetic determination of trimethoprim resistance in female patients suffering from vaginal infections

الملخص EN

During the study period,45 samples were taken from female patients who complaining from vaginal infections.

Cultural and biochemical characterization were performed followed by using api -20 E system for more accurate diagnosis.

Results showed that out of the total number of samples, 36 isolates belonged to Enterobacteriaceae.

In order to determine the susceptibility of the isolate to trimethoprim, disk diffusion method was used where results showed that 10 of 16 Escherichia coli isolates were resistant, all five Proteus mirabilis isolates were also resistant, while 2 of 5 isolates of Enterobacter cloacae were resistant, and 7 isolates of Klebsiella pneumoniae were susceptible, 3 were resistant and 1 isolate was intermediate.

Genetic detection of distribution of dfrA7 gene among isolates were performed by polymerase chain reaction technique.

Most E.coli possessed this gene, only 2 isolates of P.

mirabilis, 3 of E.cloacae, and K.pneumoniae with one isolate.