The possible effect of omega 3 fatty acids against cardiovascular diseases in rats : biochemical and histopathological studies

الملخص EN

This research is designed for detect the possible protective effect of omega 3 poly unsaturated fatty acids (PUFA) against cardiovascular diseases (CVD) in rats.

Fifty rats were divided into five groups each group contain ten rats.

The first group, control group, received control diet without any additives.

The second group was injected intraproteneally with doxorubicin 2.5mg/kg b.wt in 6 injected equally and alternatively in 6 injections for 2 weeks.

The third group was given omega 3 fish oil 400mg/kg b.wt daily for one month via intragastric tube.

The fourth group was injected with doxorubicin as second group, and then given omega 3 fatty acids for 2 weeks.

The fifth group, was given fish oil omega 3 fatty acids for 2 weeks, and then was injected with doxorubicin as second group.

Serum samples were collected for assessment of heart muscle function through (CK, LDH enzymes).

Heart was divided longitudinally to three parts taken for antioxidant enzymes activities determination, gene expression and histopathological examination.

The result of CK and LDH concentrations in serum showed significant elevation in doxorubicin injected groups, decrease in groups received omega 3.

Superoxide dismutase (SOD), glutathione S transferase (GST) and Catalase (CAT) activities were elevated in groups received omega 3.

GSH concentration was also elevated in groups received omega 3.

The gene Rp6kb1 expression showed improvement in groups received omega 3.

Finally, we can conclude that omega 3 have a significant protective and therapeutic effect on cardiovascular diseases.