Effects of ocimum sanctum extract against biochemical aspects of spodoptera littoralis (Lepidoptera : Noctuidae)‎ larvae

الملخص EN

The methylene chloride leaves extract of Ocimum sanctum (L.) was studied against the 2nd and 4th instar larvae of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) strain.

The LC50 value of the O.

sanctum extract was lower against the 2nd instar larvae than the 4th instar and the time effect on the toxicity of the extract, where the LC50 after the 48 hrs was lower than after 24 hrs.

against the two ages of the larvae.

The biochemical aspects of S.

littoralis larvae were detected using the LC50 of O.

sanctum extract against the 2nd and 4th instar larvae.

The O.

sanctum extract exhibited variations of activities for each enzyme.

It increased the activity of AST (Aspartate Transaminase) and beta esterases, on the contrary they decreased the activity of ALT (Alanine Transaminase), alpha esterases, Acetylcholinesterase (AchE), alkaline phosphatases, total protein and total lipids in both 2nd and 4th instar larvae, while O.

sanctum extract decreased the activity of acid phosphatase only in the 2nd instar larvae and elevated it in the 4th instar larvae.

Conversely, the carbohydrates increased in the 2nd and decreased in the 4th instar larvae.

The results of the research show that the overall effects of O.

sanctum extract on some biochemical components in S.

littoralis larvae can facilitate the development of selective natural product as insecticides that can be employed in integrated pest management strategies.