A comparative study of the use of dexamethasone, N-acetyl cysteine, and theophylline to ameliorate renal ischemia–reperfusion injury in experimental rat models : a biochemical and immuno-histochemical approach
المؤلفون المشاركون
Zayd, Ahmad Salah al-Din Sayyid
Sayyid, Safinaz Salah al-Din
المصدر
Saudi Journal of Kidney Diseases and Transplantation
العدد
المجلد 31، العدد 5 (31 أكتوبر/تشرين الأول 2020)، ص ص. 982-997، 16ص.
الناشر
تاريخ النشر
2020-10-31
دولة النشر
السعودية
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Renal ischemia–reperfusion injury (IRI) is commonly encountered in clinical practice during renal transplantation.
In a trial to find the drug that best safeguards the kidney against IRI, dexamethasone (Dex), N-acetyl cysteine (NAC), and theophylline (Theo) were tested in experimental rat models.
This study included 105 adult male albino rats, which were randomly assigned to the following five groups: Group I – sham-operated, n = 5, Group II – IRI n = 25, Group III – IRI + Dex n = 25, Group IV – IRI + NAC n = 25, and Group V –IRI + Theo n = 25.
IRI was induced for 40 min followed by reperfusion.
Rats were sacrificed 1, 2, 4, 6, and 24 h after reperfusion.
This was preceded by blood and urine sampling for biochemical study of serum Cystatin C (Cys C), serum creatinine, and urinary Cys C.
Kidneys were processed for histopathological evaluation and immune-histochemical staining for Cys C.
The expression of Cys C in the proximal tubular cells was significantly lower in the IRI group compared to that of the sham group.
There was a significant rise in the levels of serum and urinary Cys C after 1 h in the IRI group, while the rise in creatinine occurred later.
Dex was superior to NAC and Theo 24 h after the IR insult, and the serum levels of creatinine and Cys C were significantly lower in this group than the other two drug groups (P <0.001 in both cases).
Our study revealed a clear benefit for the use of Dex to ameliorate IRI over NAC and Theo if used immediately following the insult.
The effect is evident 24-h after its use.
The role of serum Cys C as an early marker of acute kidney injury compared to serum creatinine is confirmed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zayd, Ahmad Salah al-Din Sayyid& Sayyid, Safinaz Salah al-Din. 2020. A comparative study of the use of dexamethasone, N-acetyl cysteine, and theophylline to ameliorate renal ischemia–reperfusion injury in experimental rat models : a biochemical and immuno-histochemical approach. Saudi Journal of Kidney Diseases and Transplantation،Vol. 31, no. 5, pp.982-997.
https://search.emarefa.net/detail/BIM-1331675
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zayd, Ahmad Salah al-Din Sayyid& Sayyid, Safinaz Salah al-Din. A comparative study of the use of dexamethasone, N-acetyl cysteine, and theophylline to ameliorate renal ischemia–reperfusion injury in experimental rat models : a biochemical and immuno-histochemical approach. Saudi Journal of Kidney Diseases and Transplantation Vol. 31, no. 5 (Sep. / Oct. 2020), pp.982-997.
https://search.emarefa.net/detail/BIM-1331675
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zayd, Ahmad Salah al-Din Sayyid& Sayyid, Safinaz Salah al-Din. A comparative study of the use of dexamethasone, N-acetyl cysteine, and theophylline to ameliorate renal ischemia–reperfusion injury in experimental rat models : a biochemical and immuno-histochemical approach. Saudi Journal of Kidney Diseases and Transplantation. 2020. Vol. 31, no. 5, pp.982-997.
https://search.emarefa.net/detail/BIM-1331675
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 995-997
رقم السجل
BIM-1331675
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر