Molecular characterization of some genotypes of pomegranate (Punica Granatum L.)‎ by RAPD marker in Sulaimani region of Kurdistan-Iraq

الملخص EN

Randomly amplified polymorphic DNA (RAPD) markers was used as a good tool to evaluate genetic diversity of pomegranate cultivars.

out of 12 random primers, only 10 primers amplified products.

primer products ranged from 6 to 10 bands.

total number of bands amplified was 82, out of which 71 were polymorphic and 11 monomorphic.

the maximum, minimum and mean values of polymorphic bands were 9, 5 and 7.1, respectively.

the pic values were recorded range between 0.77 to 0.92.

Dendrogram was constructed by using UPGMA method for the clustering for all the selections that the dissimilarity coefficients were ranged between 0.22 (g 12 vs.

g 23) to 0.75 (g 1 vs.

g 6), and 5 clusters such as (a, b, c, d and f) with a mean dissimilarity (0.54) were obtained for 24 pomegranate genotypes using 10 RADP markers.

molecular analysis showed high variation within accessions.

our data obtained in present study it can be concluded that RAPD studies can be useful in breeding programmers allowing the identification of different selections and assessing the genetic similarity among them, which would facilitate their use as identified genetic stock in future breeding programmers.