The effect of some antioxidant on the physical an biochemical characteristic of semen in awassi rams

الملخص EN

The current study was designed to evaluate the effect of some antioxidant substances on the macroscopical, microscopical and biochemical properties of semen and the concentration of some antioxidant enzymes in the seminal plasma of the awassi rams twenty-four awassi rams were used in this study.

the animals were divided into four groups (6 rams / group) ; the first group was the control group.

group II the rams were given the curcuma 3 g/kg feed, group III were administered vitamin e Supplemented orally (capsule) at a dose of 400 IU / animal, three doses per week, group IV were given the coenzyme Q10 Supplemented orally (capsule) at a dose of 400 mg / animal, three doses per week..

semen samples were collected using an electroejaculaor once every 15 days along the duration of the experiment.

semen samples were evaluated to determine the volume, viscosity, pH, mas and individual motility of sperm, percentage of live, dead and abnormal sperms, seminal plasma content of total protein, albumin, globulin, cholesterol, triglyceride and the level of MDA, AST, ALT, SOD and CAT.

the results showed a significant effect (P < 0.05) of the treatments on some semen parameters ; these includes ; the volume of the ejaculates were increased in the group of curcuma and coenzyme Q10.

the viscosity was significantly affected by curcuma treatment, while the individual motility and the percentage of live sperm were increased significantly in the three treatments groups.

vitamin e and coenzyme Q10 were decrease the percentage of sperm abnormalities in comparison with control group.

there was a limited effect of treatments on the seminal plasma components ; the level of albumin and cholesterol were decreased significantly in the group of coenzyme Q10, while the level of the globulin was increased in the three treatment groups than in control group.

there has also a significant decrease in the level of cholesterol in the curcuma group and a significant increase in the level of triglycerides in the vitamin e treatment compared to the control group.

there was a limited effect of the treatments on the enzyme activity ; the MDA was part of ph.D thesis submitted by the first author increased in the three treatment groups, increased GSH in and vitamin e groups, and increased SOD in the curcuma and coenzyme Q10 groups in comparison to the control group.

it be concluded that the addition of curcuma to feed has an improved effect of semen properties ; and increased the concentration of fructose in seminal plasma.

oral administration of vitamin e was increased the level of GSH and the globulin, also administration of coenzyme Q10 was increased the level of SOD in the seminal plasma.