Effect of autologous peritoneum and platelet-rich fibrin graft on healing of intestinal anastomosis in dogs

الملخص EN

In the present study, the effect of autogenous peritoneum graft (PG) with platelet rich fibrin (PRF) was evaluated on intestinal anastomosis healing in dogs.

eighteen local breed adult dogs were anesthetized then jejunal intestinal resection and anastomosis were created on all animals.

animals were divided into 3 equal groups (6 for each).

in first group (control): an end-to-end intestinal anastomosis was performed using simple interrupted suture pattern only.

in group PG: the anastomosis site was sutured as in control group and was wrapped with pg.

where as in PRF + PG group: the anastomosis site was sutured also as in first group and was wrapped with PRF and pg.

healing process was studied in all above mentioned groups clinically, grossly, histologically and radiographically at 15 and 30 days post operation.

results were revealed that the utilizing of PG caused a valuable influence on anastomosis site healing.

this impact was manifested by faster re-epithelization of mucosa, increased collagen deposition, fibroblast proliferation, reduced adhesions, and decreased stenosis degree comparison with control group.

also there was less adhesions, less stenosis degree, increased in epithelization of the mucosa in the group treated with PRF + PG than the group treated with PG alone.

in conclusion the current study has shown that autologous peritoneal graft application has a positive and beneficial effect on intestinal anastomosis healing.

also the results highlighted the promising effects of PRF in conjunction with PG on intestinal anastomosis healing.

the application of PRF with PG at the intestinal anastomosis site is successful, safe, and highly efficient with no complications.