Major-surface-protein-4-gene-based detection of Anaplasma marginale isolated from sheep in al-Diwaniyah province, Iraq

الملخص EN

This study was purposed for confirming detection and typing of anaplasma SPP in infected sheep from al-Diwaniyah province, Iraq.

sampling of 50 blood specimens was performed from clinically-identified infection of anaplasmosis.

the samples of the blood were subjected to DNA extraction followed by polymerase-chain-reaction (PCR)-based detection of the anaplasma marginale using major surface protein (MSP4) gene.

the results have shown that 8 blood samples were infected with a.

marginale.

the PCR-based identification has revealed a confirmative identification of the anaplasma marginale in the infected sheep.

the study identifies anaplasma marginale as a member of infectious agents that affect sheep in the study city.