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Assessment of autophagy as possible mechanism of the antitumor effects of arsenic trioxide andor cisplatin on ehrlich ascites carcinoma model
المؤلفون المشاركون
Mansur, Muhammad A.
Ibrahim, Wafa M.
Shalan, Iman S.
Salamah, Afrah F.
المصدر
Alexandria Journal of Veterinary Sciences
العدد
المجلد 61، العدد 1 (30 إبريل/نيسان 2019)، ص ص. 159-167، 9ص.
الناشر
جامعة الإسكندرية كلية الطب البيطري
تاريخ النشر
2019-04-30
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
-Arsenic trioxide (As2O3) (ATO) and Cisplatin (CIS) have potent antineoplastic effects in several types of cancer.
Autophagy is important for normal cell function and survival, it is also used by tumor cells so, and we studied its role as possible mechanism of ATO and/or CIS antitumor effect on mice bearing Ehrlich Ascites carcinoma (EAC) and checked whether ATO can enhance the antitumor potential of CIS.
The study was carried out on eight groups of female mice; GP1 (negative control), GP2 (Erlich tumor only), GP3 (Normal +ATO), GP4 (Normal + CIS), GP5 (Normal + ATO+CIS), GP6 (EAC+ATO), GP7 (EAC+CIS) and GP8 (EAC +ATO+ CIS).
In this study, viable cells were counted, where percentage of viability (%) was calculated.
Flow cytometry of autophagosome was appointed.
Glutathione S-transferase (GST) and catalase (CAT) enzymes activities, total thiol and malondialdehyde (MDA) concentrations in liver tissues were determined to evaluate the effects of these drugs.
Treatment with ATO (GP6) induced a significant decrease in tumor volume and percentage of viability with best result in combination of ATO with low dose of CIS (GP8) against EAC.
Our results showed that a reduction in fluorescence intensity of autophagosome marker that determined by flow cytometry especially in combination treated group (GP8).
CAT, GST enzymes activities and total thiol level were decreased, while MDA level was increased in ATO treated group (GP6) and CIS treated group (GP7) as compared to EAC group.
On the other hand, combining both ATO and CIS in EAC treated group (GP8) decreased MDA level and augmented the level of total thiol and activities of CAT and GST enzymes.
Therefore, our result revealed that combination of ATO and CIS have anti-tumor effects against EAC better than each one alone.
So, we recommended the combination of ATO with CIS treatment due to their synergetic effect on cancer
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Salamah, Afrah F.& Mansur, Muhammad A.& Ibrahim, Wafa M.& Shalan, Iman S.. 2019. Assessment of autophagy as possible mechanism of the antitumor effects of arsenic trioxide andor cisplatin on ehrlich ascites carcinoma model. Alexandria Journal of Veterinary Sciences،Vol. 61, no. 1, pp.159-167.
https://search.emarefa.net/detail/BIM-1347946
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mansur, Muhammad A.…[et al.]. Assessment of autophagy as possible mechanism of the antitumor effects of arsenic trioxide andor cisplatin on ehrlich ascites carcinoma model. Alexandria Journal of Veterinary Sciences Vol. 61, no. 1 (Apr. 2019), pp.159-167.
https://search.emarefa.net/detail/BIM-1347946
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Salamah, Afrah F.& Mansur, Muhammad A.& Ibrahim, Wafa M.& Shalan, Iman S.. Assessment of autophagy as possible mechanism of the antitumor effects of arsenic trioxide andor cisplatin on ehrlich ascites carcinoma model. Alexandria Journal of Veterinary Sciences. 2019. Vol. 61, no. 1, pp.159-167.
https://search.emarefa.net/detail/BIM-1347946
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 166-167
رقم السجل
BIM-1347946
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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