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Partial characterisation of two isolates of cucumber mosaic virus (CMV)
العناوين الأخرى
توصيف جزئى لعزلتين من فيروس موزايك الخيار Cucumber mosaic virus
المؤلفون المشاركون
Kawanah, Maha Adil Ibrahim
Wajih, al-Sayyid E.
Zalat, Muhammad M.
المصدر
Scientific Journal of Agricultural Sciences
العدد
المجلد 3، العدد 1 (30 يونيو/حزيران 2021)، ص ص. 144-157، 14ص.
الناشر
تاريخ النشر
2021-06-30
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الموضوعات
الملخص EN
Field observation revealed that the most common symptoms on naturally infected plants in Alexandria, Dakahliya, Kafr El-sheikh and El- Beheira governorates in Egypt were severe mosaic, mild mosaic, chlorosis, mottling, vein banding, blisters, malformation, fern leaf, shoe-string and/or stunting.
These symptoms were suspected of being caused by Cucumber mosaic virus (CMV) and confirmed by indirect ELISA.
Two samples obtained from wild tobacco and cucumber plants reacted positively with the CMV specific antiserum.
Based on symptomology and disease severity on Nicotiana glutinosa and Chenopodium amaranticolor, two isolates of CMV were named CMV-wild tobacco (from Alexandria) and CMV-cucumber (from Kafr El-sheikh) and subjected to this study.
Reaction of some diagnostic hosts of 11 plant species belonging to five families upon inoculation with the two isolates included different symptoms characteristic to CMV-infection, albeit those induced by CMV-wild tobacco being invariably more severe than those elicited by CMV-cucumber.
Identification of virus isolates was confirmed using real time reverse transcription PCR (qRT-PCR).
The test isolates demonstrated a single qRT-PCR amplification product of 500bp.
The two isolates could be transmitted mechanically and easily transmitted by four aphid species in non-persistent manner.
The most efficient vector was Myzus persicae followed by Aphis gossypii, Rhopalosiphum maidis and A.
nerii with transmission rates of 90% , 70% , 60% and 50% , respectively, for CMV-wild tobacco and being 80% , 80% , 40% and 30% , respectively for CMV-cucumber.
The two isolates could not be transmitted via Cucurbita pepo seeds derived from infected plants.
However, virus infection had a great effect on seed germination.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wajih, al-Sayyid E.& Zalat, Muhammad M.& Kawanah, Maha Adil Ibrahim. 2021. Partial characterisation of two isolates of cucumber mosaic virus (CMV). Scientific Journal of Agricultural Sciences،Vol. 3, no. 1, pp.144-157.
https://search.emarefa.net/detail/BIM-1362825
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wajih, al-Sayyid E.…[et al.]. Partial characterisation of two isolates of cucumber mosaic virus (CMV). Scientific Journal of Agricultural Sciences Vol. 3, no. 1 (2021), pp.144-157.
https://search.emarefa.net/detail/BIM-1362825
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wajih, al-Sayyid E.& Zalat, Muhammad M.& Kawanah, Maha Adil Ibrahim. Partial characterisation of two isolates of cucumber mosaic virus (CMV). Scientific Journal of Agricultural Sciences. 2021. Vol. 3, no. 1, pp.144-157.
https://search.emarefa.net/detail/BIM-1362825
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
-
رقم السجل
BIM-1362825
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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