Optimizing double repetitive pcr for molecular typing of mycobacterium tuberculosis

الملخص EN

Objective: establishing a simple, cost effective and efficient method for the molecular epidemiologic examination of Mycobacterium tuberculosis based on double repetitive element polymerase chain reaction technique. Methods: fourteen isolated and characterized Mycobacterium tuberculosis provided genomic samples for the amplification using the double repetitive element polymerase chain reaction method, the resulting DNA fragments were stained using silver staining and results were compared with the original detection method. Results: The introduction of relatively simple modifications improved significantly the efficiency of isolate stain discrimination, without rendering the method more costly. Conclusions: The proposed improved method can be expected to better serve as a molecular epidemiologic technique for the fight against the widespread mounting threat of tuberculosis in developing countries.